Effect Of Exercise Training On Expression Of Nogo-A In Infarction Boundary Zone Of Cerebral Motor Cortex And Level Of TNF-α In Serum Affter Cerebral Infarction In Rats

objective: Cerebral infarction is one kind of dangerous disease, which threaten the lives of the human race and accounted for more than 70% of stroke, it retains the high rate of incidence、mortality、disability and recrudescence. Cerebral ischemia treatment can be divided into operation therapy and non operation treatment, the exercise training is clinically used as a non operation treatment. In recent years, many experiments have shown that exercise training for cerebral ischemia is effective, it has been recognized by many scholars at home and abroad. Therefore, this article trys to explore the mechanism of exercise training treatment of cerebral ischemia model. we established rat cerebral infarction model, the training group received training intervention. All of rats were selected to do evaluations by modified neurological severity scores(m NSS), and then we observed the gross morphology changes of articular cartilage 、 the pathological changes of cartilage cells, the expression of nerve myelin-associated inhibitory factor protein-A(Nogo-A) in the cartilage cells and the lever of tumor necrosis factor-α(TNF-α) by Enzyme-linked immunosorbent assay(ELISA). It intends to explore the therapeutic effect of exercise training in animal models of rat cerebral mechanisms. Methods: 1、The methods of selecting and interring rats: Choosing 84 SD healthy male rats(250g- 330g), randomly selected 28 rats as normal group, no model of middle cerebral artery occlusion(MCAO). Webuilt the MCAO model in rats by applying Longa thread cork. The rest of the 56 rats randomly divided into the model group and the training group. After the surgery, according to the Longa assessment for behavior score, score 1-3 as the successful model were used for research. The training group received training intervention. 2 、 Outcome: ①Each group were selected to do evaluations by m NSS, 7 rats from each were taken blood and brain sample at the day 1,7, 14, and 28 post MACO procedures, and then; ③We observed the pathomorphological change of cerebral tissues through HE stain, and determined the lever of TNF-α by ELISA and expression of Nogo-A in infarction boundary zone of cerebral motor cortex by IHC. 3、Statistical methods: Statistical analysis was performed using SPSS19.0 for windows software. x ±s was used to express the result, one-way analysis of variance could be used in it and P <0.05 was statistically significant. Results: 1、m NSS: The m NSS scores of the training group and the model group after modeling 1d were gradually declining. When modeling after 1d, scores between the two groups was not statistically significant(P >0.05). The limb function was higher in training group than that in control group at 7d, 14 d, and 28 d after cerebral infarction(P <0.05). 2、HE stain observing: Regional cerebral cortex movement of The normal group, neurons in the hippocampus aligned, did not see the Pathological changes. Model group visible structure Change, the decrease in the number of cells, cells were arranged disorder. More cell were damaged, Karyopyknosis and hyperchromatic, the training group compared with model group, the former changed relatively gently. 3、Nogo-A: Analysis of the quantitative immunohistochemistry, Exercise training(7d, 14 d and 28d) can reduce the expressions of Nogo-A, The difference between the exercise training group and model group were statistically significant(P <0.05). 4、TNF-α: Compared with the normal group, the exercise training group and model group had higher level of TNF-α, the differences were significant(P <0.01); After 7 day, the model group and the training group in TNF-α level began to drop, Compared with the exercise group, the model group drop slowlier, the difference was significant(P <0.05); After 14 day, the model group and the exercise training group decreased content, the exercise group decreased faster, but the differences were significant(P >0.05); After 28 day, the level of the model group and the training group was near normal content. Conclusion: 1、Using Longa thread cork method, can simulate the process of cerebral infarction and successfully set up the MCAO model; 2、Among the cerebral ischemia rats, exercise training is an effective method to improve cerebral histopathology expression. Expression of Nogo-A in infarction boundary zone of cerebral motor cortex and the level of TNF-α in serum and the m NSS scores of the training group 3、Exercise training promote the rehabilitation of neurological function deficit, the mechanism is related to decrease expression of Nogo-A in infarction boundary zone of cerebral motor cortex and the level of TNF-α in serum.