Study On Improving The Quality Standard Of Sabina Chinensis And Juniperus Formosana

Tibetan medicine of Cupressaceae of the Juniperus genus plants’ Juniperus formosana Hayata is one of the peculiar species in China, mainly distributed in the northwest regions of China. In 《Chinese Tibetan herbal medicine》 tibetan medicine’s Juniperus formosana Hayata is called" Xiubacaijian", Its roots, trunk, seeds and leaves can be extracted to volatile oil. The roots’volatile oil contain the cedar oil hydrocarbon-. cypress incense brain,cedrylone, pine oil hydrocarbon; Leaf oil contains lemon terpene and a little of gin sesame oil hydrocarbon, etc; Seeds’volatile oil contain Isobornyl acetate and camphene, etc. Cypress incense brain which can be processed into cedryl acetate as the deodorant reservation agents, the daily flavor and perfumery raw materials, etc.; It can be used in increasing the perfumery of cosmetics, daily necessities, edible flavor and enhance the taste of food fragrance. It also can be used as the cleaner of the optical glass and the microscope lens’oil infusion, etc;The Juniperus formosana Hayata contain rich flavonoids which have some efficacy such as purging fire、detoxifying、diuretic and dispel dampness;These efficacy can treat some symptom such as nephritis, edema, gonorrhea, rheumatic pain, anthrax, trauma hemorrhage,etc; Juniper plant of wood texture is straight, uniform, meticulous structure resistance to wet which can be used to make a series of material such as bridge column, the bottom, the pile of wood, stationery, handicrafts and furniture.Tibetan medicine of cupressaceae of Sabina Mill. genus plants are mainly distributed in northwest and southwest mountain areas, resistance to cold and drought. Common Tibetan medicine of Sabina Mill plants are from Sabina tibetica Kom.、Sabina convallium(Rehd.et Wils.)Cheng. W.T.Wang、Sabina wallichiana (Hook.f.etThoms Kom.)、Sabina przewaskii Kom .and so on .Tibetan medicine of Sabina Mill. according to different resources for parker called to different names, for eample, in 《Chinese Tibetan herbal medicine》 Tibetan Sabina is called "Xiuri",but in 《the system of Chinese ethnic minorities traditional medicine》 is called "Xiu ba zhe wu" and so on. The roots and branchs contain resin and the volatile oil that have main components of pinene and cedar alcohol; Its heartwood contains cedar alcohol, Japanese thuja ene, thyme hydroquinone, carvacrol, etc;Its leaves have volatile oil which have the main components of alpha pinene and limonene etc. Tibetan Sabina have some functions such as expelling wind-damp and dissipating cold、activating blood and detumescence、detoxication and diuresis、anticorrosion and removiong dampness etc. So it can treat some symptom.For eample,oral can cure chill cold, pulmonary abscess, urinary tract infections etc,Topical treatment can cure rheumatoid arthritis, urticaria and other diseases. Sabina Mill. genus plants’wood texture is fine grain, firm is not easy to wear,so it can provide the raw material for building house and producting furniture etc.Juniperus formosana Hayata and Sabina Mill. genus plants are one of the herbs which Tibetan people use medicine. In 2010 version 《Chinese pharmacopoeia》 have been not to control Juniperus formosana Hayata and Sabina Mill. genus plants quality, and the existing literature is only to have part of item to identify Juniperus formosana Hayata and Sabina Mill. genus plants,so this article is carried out to improve quality standards for Juniperus formosana Hayata and Sabina Mill. genus plants.The main results as follows:1 Carried out the original survey for Tibetan medicine of Juniperus formosana Hayata and Sabina Mill, genus plants:to investgate the main producing area in our country of Tibetan medicine base source. Collected different places of herbs and plant specimens. To carried out field trip in Gansu Gannan,Tianzhu,Sunan and other areas and collected specimens. In Gansu Yuzhong we carried out ecology and species collection. Think that the source of domestic Tibetan medicine of Juniperus formosana Hayata and Sabina Mill. genus plants is complex. Among them Tibetan medicine of Sabina Mill. genus plants have lots of main sources, such as Sabina tibetica Kom.、Sabina convallium(Rehd.et Wils.)Cheng.W.T.Wang、Sabina wallichiana (Hook.f.etThoms Kom.)、Sabina przewaskii Kom.and so on. Establish quality standards for Tibetan medicine of Juniperus formosana Hayata and Sabina Mill. genus plants.2 Complete Tibetan medicine Juniperus formosana Hayata and Sabina Mill, genus plants’ characters and microscopic identification:Carried out identification of appearance and microscope to Juniperus formosana Hayata is from Gannan and Tibetan medicine of Sabina Mill plants are from Sabina tibetica Kom.、Sabina convallium(Rehd.et Wils.)Cheng.W.T.Wang、 Sabina wallichiana (Hook.f.etThoms Kom.)、Sabina przewaskii Kom. Supple and complete raw pharmacognosy of Tibetan medicine of Juniperus formosana Hayata and Sabina Mill plants.3 Complete Tibetan medicine Juniperus formosana Hayata and Sabina Mill. genus plants’ record of TLC statutory identification:Studyed and compared the best condition of TLC of Juniperus formosana Hayata is from Gannan and Tibetan medicine of Sabina Mill plants are from Sabina tibetica Kom.、Sabina convallium(Rehd.et Wils.)Cheng.W.T.Wang、Sabina wallichiana (Hook.f.etThoms Kom.)、Sabina przewaskii Kom. Added the TLC identification of Tibetan medicine Juniperus formosana Hayata and Sabina Mill. genus plants, provided a new technology to control the quality of It has record of Tbetan medicine Juniperus formosana Hayata and Sabina Mill. genus plants.4 Determination of Three Main flavonoids in Sabina chinensis and Juniperus formosana by HPLC:Samples were extracted in 70% methanol with ultrasonic process. The HPLC was performed on an ODS-C18 column(4.6mm×150mm,5μm) with 0.2% H3PO4acetonitrile-0.2% H3PO4 as mobile phase. The flow rate was 1.0ml·min-land the column temperature was kept at 40℃. The detective wavelength was 360 nm. The linear range of rutin was 0.0049～0.1464μg (R2=0.9998);the linear rang of quercetin-3-rhamnoside was 0.0094～0.2814μg (R2=0.9997) and the linear range of kaempferol was 0.0054～0.1620μg (R2=0.9996). The average recovery of rutin; quercetin-3-rhamnoside and kaempferol were 100.12%(RSD2.55%),96.35% (RSDO.72%),96.13% (RSD0.49%) (n=6). The results showed that three Flavonoids reached baseline separation within 40min. This method has advantages of using little analytic time, a good stability and accuracy of operation, has some references value for establishing standardization quality control method of Sabina chinensis and Junipeius formosana herb.5 The total flavonodis in Tibetan medicine Juniperus formosana Hayata and Sabina Mill. genus plants with different origins were compared with ultraviolet spectrophotometry:The total flavonodis in Tibetan medicine Juniperus formosana Hayata and Sabina Mill. genus plants were extracted by ultrasonic method, quercetin-3-rhamnoside was used as reference substance,used ultraviolet spectrophotometry method, to survey the total flavonodis in Tibetan medicine Juniperus formosana Hayata and Sabina Mill, genus plants at 500nm wavelength. The linear range was 0.002892mg/mL～0.020244mg/mL(r=0.9997). The average recovery with corresponding RSD of 1.87% were 99.46% respectively. This method stability,repeatability,recovery and corresponding can be reached study of requirement of quantitative analysis methodology, it can use to Tibetan medicine Juniperus formosana Hayata and Sabina Mill. genus plants’ quality control.6 Volatile oil composition analysis:Extraced volatile oil to Juniperus formosana Hayata of Gannan and Sabina przewaskii Kom. then compared and analyed the volatile oil. First, utilized method of pharmacopoeia to extract the volatile oil to Juniperus formosana Hayata of Gannan and Sabina przewaskii Kom. then utilized GC-MS to compare and analyze the volatile oil components to Juniperus formosana Hayata of Gannan and Sabina przewaskii Kom. Found that the volatile oil components to Juniperus formosana Hayata of Gannan and Sabina przewaskii Kom. are quite different. This study can raised questions for the further research to Tibetan medicine of Juniperus formosana Hayata and Sabina Mill. genus plants.The experiments carried systematically study to improve the quality standards of Tibetan medicine of Juniperus formosana Hayata and Sabina Mill. genus plants. Added quality control indicators such as TLC,content detennination,etc.Method which is studyed and established is accurate,simple and reliable.This experiment carried out integration,improved to the existing research of Tibetan medicine of Juniperus formosana Hayata and Sabina Mill. genus plants and provided a theoretical foundation to Tibetan medicine of Juniperus formosana Hayata and Sabina Mill. genus plants’quality control standards.