| Objective:In present study, the extraction of Piperis kadsurae caulis was selected as the research object, then we evaluated anti-inflammation, analgesic as well as anti-rheumatoid arthritic of Piperis kadsurae caulis extraction.Methods:1. The anti-inflammatory activities of Piperis kadsurae caulis extraction were oserved by utilizing the following models:carrageenin-induced paw edema in rats, xylene-induced ear edema and tampons-induced granuloma formation in mice.2. The analgesic effeets of Piperis kadsurae caulis extraction were evaluated by hot-plate test and acetic acid-induced writhing response in mice as well as chlonic pain model in collagen-induced arthritic rats.3.Collagen-induced arthritic rat model was established to evaluate the anti-arthritic effetes of Piperis kadsurae caulis extraction. We determined the change of paw swelling and histological assessment of ankle articular synovium of all CIA rats.4. Anti-rheumatoid arthritic mechanism research of Piperis kadsurae caulis extraction: the levels of the TNF-α, IL-6, et al. in synovial tissues were examined by western blot and reverse transcription-polymerase chain reaction (RT-PCR) analysis.Results:1. Anti-inflammatory experiments:(1) xylene-induced ear edema experiment:the ear weigh of Piperis kadsurae caulis extraction groups all were less than the model, and there were significant difference compared with the model group (P<0.05 or P<0.01);(2) Cotton pellet granuloma formation experiment:granulation tissue weigh of Shuangtengbitong tincture groups all were less than the model, and there were significant difference compared with the model group (P<0.05 or P<0.01);(3) Carrageenin induced foot pad swelling experiment:foot pad swelling of Shuangtengbitong tincture groups all were less than the model, and there were significant difference compared with the model group only in Piperis kadsurae caulis extraction high-dose groups at 2 h,4 h,6 h;2. Analgesic experiments:(1) The hot plate experiment:the pain response time of Piperis kadsurae caulis extraction groups were prolonged, Piperis kadsurae caulis extraction high-dose groups showed significant effect;(2) Chemical irritants experiment:the pain response time of Piperis kadsurae caulis extraction medial-dose and high-dose groups were prolonged compared with the model group, and torsion times of mice within 15 mins was obviously reduced (P<0.05 or P<0.01).3. Effect on CIA rats experiments:Paw swelling of Piperis kadsurae caulis extraction groups were less than the model group. And also improved the inflammatory response in synovial tissues. High-dose group had significant difference compared to the model group.4. Anti-rheumatoid arthritic mechanism researchProtein and mRNA expression levels of TNF-α, IL-6, IL-10, NF-κB, IKκ, IκB and P-IκB were less than the model group, and high-dose group had significant difference compared to the model group (P<0.01). However protein and mRNA expression level of IκB was up-regulated and high-dose group had significant difference compared to the model group (P<0.01).Conclusions:1. Piperis kadsurae caulis extraction could significantly inhibite the inerease in Paw edema induced by carrageenin, ear edema induced by xylene and granuloma induced by agar.2. Piperis kadsurae caulis extraction obviously prolonged the writhing latent period and reduced writhing time in acetic acid-induced mice. Moreover, Piperis kadsurae caulis extraction also decreased hot-plate pain threshold.3. Piperis kadsurae caulis extraction could remarkably inhibite paw swelling, regulate the change of synovial tissues. Piperis kadsurae caulis extraction also significantly regulated inflammatory signal transduction networks and related factors, which manifested as reducing levels of serum TNF-α, IL-1β, IL-6, NF-κB, IKκ, IκB and P-IκB. It may be the anti-rheumatoid arthritic mechanism of Piperis kadsurae caulis extraction. |
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