| Objective: Biological behavior of colorectal cancer(CRC)has a complex, easy to relapse and metastasis and resistance to chemotherapy drugs, etc. Therefore, as far as possible to clarify the mechanism of colorectal cancer progression, Seek early diagnosis and effective treatment methods, and the development of rational strategies in colorectal cancer has been the focus of research in the field of experimental. Micro RNAs(mi RNAs) are an expression in a variety of eukaryotes, size is about 18-25 nucleotides non-coding small RNA molecules. Present study suggests that mi RNAs may be involved in cell growth, differentiation, proliferation and apoptosis. Currently the human genome has identified mi RNA approximately 500, of which at least 200 kinds of cancer related mi RNA sequences.micro RNA-155(mi R-155) is located on human chromosome 21q21, is integrated by the B cell gene cluster three exons encoding the highly conserved region.mi R-155 is one of many mi RNAs, therefore, to explore mi R-155 in tumorigenesis, the relationship between the development may be an early cancer detection and early treatment of new ways. Therefore, the development of this experiment is to verify the role of mi R-155 mechanism occurs in colorectal cancer.Methods : Primary CRC tissue and matched normal adjacent colorectal mucosa were obtained from 76 patients who underwent colorectal cancer resection without pre-operative treatment at the Gastrointestinal Surgery, The Affiliated Hospital of North Sichuan Medical College, Nanchong. The expression by Cell culture, quantitative real-time reverse transcriptase-PCR(q RT-PCR),Transfection of mi RNA, MTT, migration and invasion assays,Western blot analysis, Analysis of the relationship between mi R-155 colorectal cancer occur, the development of the role and clinical pathology.Results: It was significantly upregulated in the cancer tissues from patients with CRC compared to those from the normal adjacent Mucosa,the high expression of mi R-155 significantly correlated with an advanced TNM stage, lymph node and distant metastasis(P <0.05, Table 1). However,there was no significant correlation between mi R-155 expressionand other clinicopathological characteristics, such as age, gender, histological type, tumor size, depth of invasion,tumor location.2.mi R-155 modulates migration and invasion but not proliferationin CRC cells.3. The high expression of mi R-155 enables adhesion between cells and morphological changes occurred, which was conducive cell migration and invasion。4.Regulation of reduce socs-1 expression by mi R-155 in CRC cells.Conclusion: the overexpression of mi R-155 Plays an important role in the migration and invasion of CRC. The overexpression of mi R-155 enables adhesion between cells and morphological changes occurred. its effects on CRC cells are possibly associated with the regulation of the socs-1/ZEB-1/E-cadherin axis. The results from this study provide important clues as to the mechanisms involved in migration and invasion in CRC... |
PDF Full Text Request