The Knock-down Of USP7 Changes The Biological Characteristics Of Laryngeal Cancer HEP2 Cells

Objective: The ubiquitin-specific protease 7(USP7) is associated with the pathological process of tumors. However, the role of USP7 is still not clear in laryngeal cancer cells, so we knocked down USP7 by si RNA to observe the biological characteristics of laryngeal cancer HEP2 cells with the purpose of exploring the role of USP7 in laryngeal cancer.Methods: Immunohistochemistry was used to investigate the expressions of USP7 in 73 laryngeal cancer tissues and 52 para-cancer tissues. The designed most effective si RNA was selected to knock down USP7 protein expression in laryngeal cancer HEP2 cells, and the capacity of cell proliferation and migration, as well as including apoptosis, was further assessed through carrying out multifaceted experiments.Results: The expression of USP7 in laryngeal carcinoma tissues is significantly higher than that in para-carcinoma tissues(83.6% vs. 36.5%, p<0.0001). The data showed that there is the negative association between USP7 positive expression and tumor differentiation(p = 0.0479), which suggests that lower level of differentiation, higher positive expression of USP7 protein. Notably, patients with more advanced T stage showed higher expression of USP7 protein in laryngeal cancer samples, but there is no statistical significance. Furthermore, the designed si RNA-USP7 significantly inhibited the expression of USP7 m RNA. As expected, the selected effective 2# si RNA markedly suppressed the USP7 protein expression in laryngeal cancer HEP2 cells, which also inhibited the cell proliferation and migration, and further lead to increase of the cell apoptosis in laryngeal cancer HEP2 cells in vitro.Conclusions: The USP7 positive expression is higher in laryngeal cancer tissues, and is to be negatively associated with differentiation of tumor. The si RNA which can significantly knock down the expression of USP7 in laryngeal cancer HEP2 cells, inhibits the capacity of the cell proliferation and migration, and enhances the cell apoptosis in vitro. These data suggest that USP7 may be a promising therapeutic target for patients with advanced laryngeal cancer.