Expressions Of GDNF,GFAP And CD11b In Nigra In Rats With Parkinson’s Disease And The Study On Pharmacological Interventions

Objectives By observing the expressions of TH and GDNF, GFAP, CD11b in nigra inrats with Parkinson’s disease and the effects of Eldepryl on that expressions, to discussthe function of GDNF and inflammation in PD and the therapeutic mechanism ofEldepryl.Methods1. The SD rats were randomly divided into normal group, model group andEldepryl treatment group, and each group was divided randomly into4d and8dsubgroups after the success of model preparation.(Each subgroups has12rats,amongwhich6rats were used for Immunohistochemistry, others were used for western blot).2.PD model in rats was established by rotenone subcutaneous injection.3. Gavage Eldepryl(0.5mg/kg)4d and8d after model successfully administered.4. Determination of ratswith behavioral changes in disability ratings; The expression of TH and GDNF, Gliacyteswere detected by Immunohistochemistry and Western blotting.5. We observe tissueslices with microscope camera OLYMPUS(400×), then analysis them by Motic Med6.0digital medical image analysis system. The results of Western Blot were analyzed withImage J.6. Application SPSS13.0statistical software for data analysis at last.Measurement data are presented as mean±SD(x s), using anova design analysis ofvariance within the group, P<0.05for the difference was statistically significant.Results1. There were much expression of TH positive cells and expression of GDNFpositive cells in nigra in rats in normal group, and there was no significant differencebetween8d and4d(all P>0.05). The expression of TH positive cells and GDNF positivecells were all significantly reduced in model group compared with those in normal group,and there was significant difference between them(all P<0.05). There were lessexpression of TH positive cells and GDNF positive cells at8d compared with those at4d,but it has no significant difference(all P>0.05). The expression of TH positive cells andGDNF positive cells were all significantly increased in Eldepryl treatment groupcompared with those in model group(all P<0.05), and there were more expression of THpositive cells and GDNF positive cells at8d compared with those at4d, and it hassignificant difference(all P<0.05).2. GFAP and CD11b positive cells were all in a restingstate in control group, GFAP-positive cell body was slender and irregular and has elongated protrusions, CD11b-positive cell body is small, and was a branch-like, it hasmore slender protrusions. GFAP and CD11b positive cells were all in a active state,GFAP-positive cell body was hypertrophy, projections increased thickening, CD11b-positive cell body was more bigger, projections were shorter and thicker, and the numberhas increased; Compared with model group, GFAP-positive cell body and protrusionswere more slender, CD11b-positive cell body was more smaller, projections were moreslender, and the number has decreased in Eldepryl group. There were a small amount ofexpression of GFAP and CD11b positive cells in nigra in rats in control group, and therewas no significant difference between8d and4d(all P>0.05). The expression of GFAPand CD11b positive cells was significantly increased in model group compared withthose in control group, and there were significant difference between them(all P<0.05),and there were more expression at8d compared with those at4d, but it has no significantdifference(all P>0.05). The expression of GFAP and CD11b positive cells weresignificantly reduced in Eldepryl group compared with those in model group(all P<0.05),and there were less expression at8d compared with those at4d, and it has significantdifference(all P<0.05).Conclusions1. The expressions of TH and GDNF in nigra of the rats model of PD wasdecreased, well MG and As were proliferative and activated.2. Eldepryl could increasethe expressions of TH and GDNF and inhibit the proliferation and activation of MG andAs.