| Objectives To explore the mechanism of CO2pneumoperitoneum on the function ofovary rats.Methods250adult female rats were divided into three groups, the control group,negative control group and the blank control. After being inflated1h,2h under the15mmHg and20mmHg pressure, and then tested the E2，P，FSH and LH in bloodserum on0h,6h,12h,and24h after the end of pneumoperitoneum. After that, took one ofthe ovaries as examples to find the changes of MDA and SOD,and the other ovary wasused for HE staining.Results1The change of E2and P of the serum of the rats: After being inflated1h, afterthe serum levels of the pneumoperitoneum rat were decreased significantly comparedwith the control group (p <0.01); When the veress ended after6h, the pneumoperitoneumrat were decreased significantly compared with the group after the pneumoperitoneumended0h; After the pneumoperitoneum in12h, serum levels of the pneumoperitoneumrat were decreased significantly compared with6h(p <0.01), they compared with thecontrol group after24h, pneumoperitoneum end0h,6h,12h decreased significantly (p <0.01);the change under pressure of20mmHg were the same of the pressure of15mmHgand the change were more apparently.(p <0.05);2The change of FSH and LH of theserum of the rats: After being inflated1h, after the serum levels of thepneumoperitoneum rat were increased significantly compared with the control group (p<0.01); When the veress ended after6h, the pneumoperitoneum rat were increasedsignificantly compared with the group after the pneumoperitoneum ended0h; After thepneumoperitoneum in12h, serum levels of the pneumoperitoneum rat were increasedsignificantly compared with6h(p <0.01), they compared with the control group after24h,pneumoperitoneum end0h,6h,12h increased significantly (p <0.01).3The change of theSOD in the rats’overian tissue: After being inflated1h, after the serum levels of thepneumoperitoneum rat were decreased significantly compared with the control group (p<0.01); When the veress ended after6h, the pneumoperitoneum rat were decreasedsignificantly compared with the group after the pneumoperitoneum ended0h; After thepneumoperitoneum in12h, serum levels of the pneumoperitoneum rat were decreased significantly compared with6h(p <0.01), they compared with the control group after24h,pneumoperitoneum end0h,6h,12h decreased significantly (p <0.01);the change underpressure of20mmHg were the same of the pressure of15mmHg and the change weremore apparently.(p <0.05).;4The changes of MDA of the overian tissue of rats’: Afterbeing inflated1h, after the serum levels of the pneumoperitoneum rat were increasedsignificantly compared with the control group (p <0.01); When the veress ended after6h,the pneumoperitoneum rat were increased significantly compared with the group after thepneumoperitoneum ended0h; After the pneumoperitoneum in12h, serum levels of thepneumoperitoneum rat were increased significantly compared with6h(p <0.01), theycompared with the control group after24h, pneumoperitoneum end0h,6h,12h increasedsignificantly (p <0.01), the change under pressure of20mmHg were the same of thepressure of15mmHg and the change were more apparently.(p <0.05).5Afterpneumoperitoneum between0h visible edema interstitial cells, cell volume increases, thecytoplasm loose; The greater the pressure, the more obvious cell edema.Conclusions1The E2, P of the rats’ serum at the same pressure and end prolongedprolonged decline Veress formed with; And the greater the pressure, the more obviousdecline;2The FSH, LH of the rats’ serum at the same pressure over prolongedpneumoperitoneum and prolonged increase in the formation of the end time;3The SODlevels in rats’ ovarian tissue at the same pressure and end prolonged decrease withprolonged pneumoperitoneum form; pneumoperitoneum with different pressures and endforming a prolonged decline, and the greater the pressure, the more obvious decline; theMDA content at the same pressure and end prolonged increases with prolongedpneumoperitoneum form; pneumoperitoneum with different pressures and end forming aprolonged prolonged and increased, and the greater the pressure, the more obvious rise.4Under the same pressure, the longer form pneumoperitoneum,, theca cells edema,Structural changes in the above20mmHg pressure was significantly higher than15mmHg. |
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