The Correlation Of Infiltration Of Regulatory T Cells And TGF-β1, IL-10Expression With Human Brain Glioma Recurrence

Objective：Detection glioma tissue CD4+Foxp3+regulatory T cells (Treg) infiltration andTGF-1, IL-10expression, analysis of the relationship between the three tumormicroenvironment to explore Treg infiltration and TGF-1,IL-10expression in differentlevels of human glioma tissues recurrence.Methods：Collect26patients (patients were excluded from inflammation, autoimmunedisease)with recurrence of brain glioma from our department these patients were involvedwith surgical resection and diagnose by pathology. Taking two glioma tissue samples fromeach patient after twice surgery, which is divided into the primary group and the recurrencegroup,according to the2007WHO classification and grading of central nervous systemtumors standard. WHO I-II level for low-grade glioma, WHO III-IV-class high-grade glioma.Take exception to the normal brain tissue injury under reduced pressure tissue blocks for thecontrol group. All tissue specimens after resection of0.5-1.5h saved in two parts, the part isplaced directly in liquid nitrogen saved for tissue protein extract, and another portion fixedwith10%formalin for paraffin. The specimens using immunohistochemistry and ELISA wasused to detect TGF-1, IL-10, Treg (CD4+Foxp3+) expression.Results：TGF-1, IL-10in glioma tissue sections distribute with cluster-shaped,Treg cells inglioma tissue sections were scattered invasion. TGF-1, IL-10and Treg in the primary groupand the recurrence group were expressed and invasion, and showed a gradual increase, whilethe control group did not express and invasion. TGF-1, IL-10expression and Treg invasiondifference between the control group and the primary group were statistically significant (P<005),the same trend exist between and the control group and the recurrence group. Thegroup of glioma relapsed pathological levels tended to increase (P <005); in the group ofglioma before and after the recurrence, the paired comparison with the expression of TGF-1,IL-10and invasion of Treg statistically significant (P <005); the expression of TGF-1,IL-10and invasion of Treg in the recurrence group than the primary group is a rising trend (P<005). The expression of TGF-1, IL-10and invasion of Treg in Glioma tissue andpathological level was positively correlated (P <005). The expression of TGF-1, IL-10andinvasion of Treg among pairwise mutually positive correlation (P <005). In the glioma tissueprotein in the supernatant, TGF-1, IL-10in the primary group and the recurrence group were detected, both of their expression were increased step by step with the pathological level;both of their expression levels difference between the control group and the primary groupwere statistically significant (P <005),the same trend exist between and the control groupand the recurrence group (P <005); in the group of glioma before and after the recurrence,theexpression of TGF-1,IL-10by paired comparison was statistically significant (P <0.05), theexpression of TGF-1, IL-10in the recurrence group than the primary group is a rising trend(P <0.05).The expression of TGF-1, IL-10were positive related with pathological level(P<0.05); the relationship between the expression of TGF-1, IL-10also positivelycorrelated (P <005).Conclusion: The expression of TGF-1, IL-10and invasion of Treg in glioma tissuemicroenvironment were increasing trend, while Treg in filtration may be associated with theexpression of TGF-1, IL-10. the evaluation of glioma progression and prognosis of immuneparameters，the three factor may be involved in promoting tumor immune escape and immunetolerance,thus avoiding immune destruction to promote malignant glioma and recurrence.