Research On HPLC/DAD Fingerprints Of Radix Astragali From Different Origination And Spectrum-effect Relationship Of Total Flavoniods In Astragalus

Objectives To determine the correlation between the chemical compositionsinformation which was reflected by lots of chromatographic peak and restores vital energy/anti–inflammatory effect. HPLC fingerprints of Radix astragali samples from different originwere established, and the spectrum-effect relationship of Astragalus flavonoids (AFS) wasinvestigated.This research shows that the proposed method can be effectively applied to trendforecast analysis and supported scientific data in term of confirm multiple active ingredient,and establish firmly basic for improving overall quality control of Radix astragaliMethods (1)12batches Radix Astragali samples come from different origins wereanalyzed with HPLC/DAD, and the chromatographic conditions was optimized. Themethodology was inspected, and the chromatographic fingerprint of Radix astragali. wasestablished. In the end, similarity evaluation of Radix Astragali fingerprint was performedwith “Chinese traditional medicine chromatographic fingerprint similarity evaluationsoftware”.(2) To enrich and purify the Astragals flavonoids (AFS) of6batch Radix astragalisamples which collected from Longde, Pengyang, Yuanzhouqu, Hongsipu, Mazichuanxiangand Meixiangzhen with D101macroporous adsorptive resins. Six pieces AFS Enrichmentparts was obtained.(3) Pharmacodynamics study of enrichment parts: Anti-inflammationeffect was determined by using of the mouse ear swelling model which caused by xylene.The restores vital energy effect of AFS was valued by the hypoxia tolerance of mouse.(4)Method of grey relational analysis was usedon the research of correlation between the chemical compositions information of AFS with restores vital energy and with anti-inflammatory effect. Finally, it was confirmed that the chemical composition which wasrepresented by fingerprint contribute to the pharmacodynamics effect according to the degreeof above correlation.Results (1) The standard chromatographic fingerprints were established by using ofmedian method, and there were fifteen common peaks were affirmed by” Chinese traditionalmedicine chromatographic fingerprint similarity evaluation software”. The similarity of12different origin of Radix astragali samples which collected from Zhaolou, Liancai, Liancainorth-mountain, Yangping, Jianjing, Xinmin, Kangxian, Kaichengliang, Shatang, Qingliang,Chengxiang, and Longxi were respectively0.850,0.966,0.946,0.884,0.980,0.978,0.987,0.951,0.920,0.901,0.905and0.858.(2) It was obtained that6batches AFS enrichment partsof Radix astragali which are indigenous to Longde, Pengyang, Yuanzhouqu, Hongsipu,Mazichuan and Meixiang by use of D101macroporous adsorptive resins. The weight of6batches AFS enrichment parts were5.92g,4.99g,6.21g,5.66g,4.84g and5.63g respectively.The yield of them were11.84%,9.98%,12.42%,11.32%,9.68%and11.26%. At the sametime, the fingerprint peaks of enrichment parts were not arisen after the experiment began in38minites.(3) During the experiment of mice ear-swollen induced by xylene,6groups ofAFS enrichment parts which were from Longed, Pengyang, Yuanzhouqu, Hongsipu,Mazichuan and Meixiang had significant difference with model group (P<0.05). The resultshowed that AFS enrichment parts have a certain anti-inflammatory activity. Within thehypoxia tolerance test,6groups of them have significant difference with model group(P<0.05). The results indicated that AFS enrichment parts have arestores vital energy effect aswell.(4) After analyzed the data of chromatographic fingerprints and pharmacodynamics ofAFS enrichment parts through grey relational analysis, The following conclusions were drew:First, The anti–inflammatory effect of AFS of Radix astragali was a composite effort of allthe chemical composition in the plant because all of the correlation between fifteen common peaks and anti–inflammatory effect is larger than0.6. Based on the results of correlationwhich are more than0.92to determine the composition of the “anti–inflammatory effect”contributed to the size of the order: Onospin>The material of retention time of21.6min>Calycosin glycoside. Second, all of the correlation between fifteen common peaks andrestores vital energy effect is larger than0.6, too. The results also indicated that the restoresvital energy effect of AFS of Radix astragali is a composite effort works by its groupchemical compositions. The size of contribution to the following order: Onospin> Calycosinglycoside> the material of retention time of21.6min.Conclusion (1) The chromatographic fingerprints of Radix astragali from12differentbatches were established, the methodology was inspected. The experimental results testifythat the approach was simple, reliable, feasible and efficient.(2) AFS enrichment parts ofRadix astragali were effectively enriched and purified with D101macroporous adsorptiveresins.(3) The result of pharmacodynamics study indicated that all AFS enrichment parts ofRadix astragali had a certain anti-inflammatory activity. The most important thing was thatthe restores vital energy effect of AFS enrichment parts of Radix astragali was firstdiscovered.(4) It confirmed that the contribution of AFS enrichment parts of Radix astragalito the effect of anti–inflammatory and restores vital energy with grey relational analysis.Furthermore, it showed that the pharmacodynamics effect of AFS of Radix astragali wasassociated with its group of chemical composition in this paper.