| PART1The establishment of the alternative high output tissue microarray technique BackgroudTissue microarray (TMA) is a high throughput research tool, which has greatly facilitated and accelerated in situ tissue analyses. However, its productivity has been restricted due to the confined thickness of traditional donor block. Here, we introduce an improved high output TMA method that is applicable to a broader range of tissue samples. ObjectiveTo establish an alternative high output tissue microarray technique. Materials and methods1)150cases of GISTs were retrospectively collected from the Department of Pathology in Zhongshan Hospital, Fudan University from2001to2012.2) The new tissue microarray technique was used to establish the tissue microarrays of70cases of GISTs and some imatinib-resistance specimens were collected. CD117immunohistochemical staining and c-KIT, PDGFR alpha gene detection were made on the tissue microarrays.3) A composite tissue microarray was made with the new technique and the traditional way. A Serial sections were made and the numbers of slice were recorded when tissue core was missing.Results1) All TMA rods were prepared as described above. The getting rate of the desired area was100%in these sections. Morphology of all spots in the first section and the last one was consistent with that in the original slides.2) Up to1500consecutive sections of3μm could be easily gained by a trained histotechnologist. The prepared TMA could be sectioned repeatedly. These sections were adhered to a special slide and stained with H&E. No missing spot was found after1500sections, while a first missing spot was presented after100sections on the TMA which was made by the traditional method.3) In the third TMA, it was possible to add new cases to the tissue microarray with the new TMA technique.4) During the period of inventing our new methods for making TMA, we applied for eight patents and five were authorized.Conclusions1) The procedure and facility are simple, omitting hole-punching step, and no special, expensive instrument is required.2) The IHC staining and DNA sequencing results showed that the TMA tissue maintained its antigens and DNA after the rods of tissues being planted in the recipient block.3) The most improvement of the method is its high production.PART2The application of the high output tissue microarray technique1Thyroid transcription factor-1expression in breast carcinomas and its potential significancePurposeThyroid transcription facor-1(TTF-1) was one of the immunohistochemical markers most commonly used in assisting in the differential diagnosis of carcinomas of the lung and thyroid as its expression was highly restricted to the thyroid cancers and pulmonary adenocarcinomas. This study was to investigate the frequency of TTF-1expression in breast carcinomas and the significance in the diagnosis and differential diagnosis of the tumors.MethodsThe expression of TTF-1in70breast carcinomas was detected by the technique of tissue chip and immunohistochemical staining. ResultsTTF-1expression in tumor cell nuclear was identified in2cases (2.9%) which were diffuse and strong. One was invasive ductal carcinoma (grade2) and the other one was invasive lobular carcinoma.ConclusionsA small proportion of breast carcinomas show TTF-1expression. The presence of TTF-1immunoreactivity cannot completely rule out a breast origin in a metastatic carcinoma.2Immunohistochemistry study in esophageal neuroendocrine carcinoma and squamous cell carcinoma by the new TMA techique PurposeTo explore new immunohistochemical parameters in the differentiating between primary neuroendocrine carcinoma (NEC) and squamous cell carcinoma (SCC) of the esophagus. MethodsThe expressions of HCK, P63, TTF-1, CD117, CD56, CHG, SYN, SSR2, SSR5and P16in primary NEC and SCC of the esophagus were detected by immunohistochemical staining in the TMA made by the new TMA techique. Results1) P16detection was proved no application value in differential diagnosis in the esophageal neoplasms.2) TTF-1only expressed in neuroendocrine carcinoma(60%), and CD117expression in SCC with NED(40%), and SCC(5.3%), but less than that in NEC (95%) in the scope of positive, positive rate, and positive strength.3) Positive rates of SSR2in esophageal NEC, SCC with NED, SCC were40%,40%and19.3%, respectively, while SSR5were70%,70%and15.8%, respectively.Conclusions1) P16were diffuse and strong expression in esophageal neoplasm (100%).2) TTF-1and CD117could be used as new immunohistochemical indicators in the diffenentiation diagnosis between NEC and SCC of the esophagus.3) SSR2and SSR5expressed in the above-mentioned esophageal carcinoma, which provide potential targets for anti-tumor role by somatostasis.PART3The transformation and promotion of the high output tissue microarray technique... |
PDF Full Text Request