A Case-control Study Of Methylation Level And Related Factors Of P16Gene, RASSF1A Gene In Breast Cancer In Xinjiang

Objective:The aim of this study was to investigate the risk factors in breast cancer in Xinjiang and the aberrant methylation of RASSF1A gene and P16gene in the plasma of patients with breast cancer.Methods:1:1case-control was adopted and the tumor suppressor gene RASSF1A and P16gene was amplified cancer and110healthy individuals, and followed the Pyrosequencing to detect the methylation of the gene by polymerase chain reaction (PCR) in the peripheral blood of110patients with breast cancer. Result:1. Univariate logistic regression analysis showed that dysmenorrheal abortion is a risk factor for breast cancer (P<0.05);Physical exercise,outgoing personality is a protective factor for breast cancer (P<0.05).2.Univariate analysis showed that the difference of methylation degree of RASSF1A PM00104139CpG-2,CpG-5was statistically significant in Case-control group(P<0.05).The difference of methylation degree of RASSF1APM00104139CpG-3and P16PM00139972CpG-2was statistically significant according to the ER-negative-positive group. The difference of methylation degree of P16PM00139972CpG-4was statistically significant according to the HER-2-negative-positive group and TNBC group(P<0.05).3.The degree of methylation difference of P16gene was negatively correlated with ER and TNBC(r=-0.027,P<0.05; r=-0.4,P<0.05).4.The degree of methylation of RASSF1A PM00104139CpG-4was negatively correlated with personality and The degree of methylation of P16PM00139972CpG-1was positively correlated with physical activity(r=-0.321,P<0.05).Conclusion:By detecting the abnomal mathylation of each P16gene and RASSF1A gene in Xinjiang breast cancer patients may be make sence to the prognosis and early prevention of breast cancer.