Anti-tumor Effect And Mechanism Study Of Ndv Cooperated With Klt In H₂₂ Liver Cancer

Cancer biotherapy has become the popular research in the cancer treatment. Newcastledisease virus (NDV) has been widely confirmed as a biotherapy which has been applied toclinical trial all over the world, but its antitumor mechanism is not fully clear. Studies showedCytokines can be induced by NDV, such as tumor necrosis factor (TNF), interferon (IFN),interleukin (IL) and so on. Kanglaite injection (KLT) is a kind of antitumor Chinesetraditional medicine preparation and has a good clinical application. We established H22livertumor models. NDV, KLT and NDV+KLT were used to cure the tumor-bearing mice. Duringthe period of the treatments, We observed the mice living condition and the tumor growth. Bymaking paraffin sections, we observed the pathological changes in tumors and other tissuesincluding heart, liver, spleen, lung, kidney and brain using H.E. staining. We checkedexpression of TNF-α, IFN-γ and IL-6of immunohistochemical. We examined the percentageof phagocytosis in the test of macrophages swallowing chicken erythrocytes to checkimmunologic function of NDV and KLT. The results showed:1. H22liver tumor models were established successfully, and the symptoms ofposttreatment in tumor-bearing mice were observed. The results showed that the diet, mentalstate, activity and the color in treatment groups were better than the control group. It indicatedthat three kinds of medication had no adverse reaction but can improve the growth andmetabolism of the tumor-bearing body. All of these data including tumor weights, tumorvolume, body weights and spleen weights were detected respectively. Compared with thecontrol group, the result showed that NDV, KLT and NDV+KLT all had inhibitory effect ontumor growth, and NDV+KLT showed a most distinct effect.2. Comparing with the control group, HE staining result showed that the tumor invasiveregion of treatment groups had a larger number of inflammatory cells infiltration andinflammatory cells formed a wider band. Tumor tissue interaction region had a large numberof immune cells and tumor cellar pieces, tissue fiber structures were complete. Tumor cellswere further spares and atypia was lower in the tumor tissue growing region. Thus, NDV andKLT had obvious effect on inhibiting tumor growth, and promoting tumor cell necrosis,especially in NDV+KLT group, which had remarkable antitumor effect in vivo.3. In tumor tissues, comparing with the control group, Immunohistochemical results showed that TNF-α, IFN-γ, IL-6were in strong positive expression in cancer cells dead zoneand were weak expressed in cancer cells thrive area. But they played weak positive expressionor negative expression in the posttreatment periods. This indicated that the antitumor effectsof these three medicines were highly related to the effect of cytokines. Macrophagephagocytosis rate the statistics of Macrophage phagocytosis rate showed that control grouphad a stable phagocytic rate in the different stages of treatment. Comparing with the controlgroup, the phagocytic rates were significantly improved. This indicated that all these threedrugs can induce and improve the body’s immune system function and play the antitumoreffect by activating immune cells to inhibite or kill tumor cells.In summary, NDV and KLT were able to improve the growth and metabolism oftumor-bearing mice, enhance the immune system function, and inhibit tumor growth. Theanti-tumor effects of NDV and KLT were highly related to TNF-α, IFN-γ and IL-6, andNDV+KLT had the most significant inhibitory effect on tumor treatment.