Based On Inflammation To Study The Mechanic Of The Treatmeat To Athersis With Guan Xinping

Research purposesFirst,to establish apolipoprotein E knockout mice atherosclerosis (CAS) model.second,to treat apolipoprotein E knockout mice coronary atherosclerosis (CAS) model with Guan Xinping and to study its pro-inflammatory and anti-inflammatory cytokines. Research contents and methods66ApoE-/-mices were divided into6groups. In addition to11that were put into the normal control group were fed with normal diet, the remaining55were fed with high fat diet (78%basal diet,2%cholesterol,10%egg yolk powder,10%lard preparation) for12weeks.Later we must do blood test and watch pathological to make sure the apolipoprotein E knockout mice atherosclerosis AS models have been.The normal control group were fed with based normal diet,, and the remaining group were fed with high-fat diet. In addition to the normal group and model of coronary flat low-dose group, Guan Xinping high-dose group, atorvastatin group, the amlodipine group of high-fat diet for12weeks were given medicines, but the normal group and model group were given the same amount of distilled water after12weeks. The drug is dissolved in distilled water and then Intragastric Guan Xinping low dose group and atorvastatin groupvolume amount calculated by O.lml/lOg into Guan Xinping low dose group and atorvastatin group once a day for a total of one month. Weighed once a week, record weight, after the last administration lh, orbital blood, serum and plasma were taken home at-20℃tested. The aortas were harvested and were stored in liquid nitrogen, to equipment test3.results3.1Guan Xinping on apoE-/-mouse atherosclerosis in aortic pathologyCompared with normal group (A), it is clear that the vessel wall damage occurs, elastic fiber breakages, endothelial cell defects in Model group (B). After giving Guan Xinping to intervent,we can find vascular morphology improved, a slight improvement in arterial wall morphology inatorvastatin group (C), amlodipine group (D), Guan Xinping high-dose group (F), Guan Xinping low-dose group (E) compared with the model group.But still partially visible continuous elastic fibers.3.2Guan Xinping on apoE-/-mouse3months on high fat in expressing ICAM1, ICAM1mRNA(1)Compared with the control group,Model group apoE-/-mice aortic tissue was significantly elevated ICAM1(P<0.01). After the treatment of atorvastatin, amlodipine, low-dose Guan Xinping, high doses of Guan Xinping, apoE-/-mouse’s ICAM1in aorta tissue levels were lower.Guan Xinping high dose group and atorvastatin group apoE-/mice ICAM1aortic tissue content is reduced, by comparing with the model group, a significant difference (P <0.01). These results suggest that Guan Xinping can inhibit the apoE-/-mouse aorta tissue ICAM1expression.(2) Compared with the control group,Model group apoE-/-mice aortic tissue was significantly elevated ICAM1mRNA (P<0.01). After the treatment of atorvastatin, amlodipine, low-dose Guan Xinping, high doses of Guan Xinping, apoE-/-mouse’s ICAM1mRNA in aorta tissue levels were lower.In Guan Xinping high dose group, amlodipine group and atorvastatin group apoE-/mice ICAM1mRNA aortic tissue content is reduced, by comparing with the model group, a significant difference (P<0.01). These results suggest that Guan Xinping can inhibit the apoE-/-mouse aorta tissue ICAMlmRNA expression.3.3Guan Xinping on apoE-/-mouse3months on high fat in EL AM1、ELAM1mRNA expression(1) Compared with the control group,Model group apoE-/-mice aortic tissue was significantly elevated ELAM1(P<0.01). After the treatment of atorvastatin, amlodipine, low-dose Guan Xinping, high doses of Guan Xinping, apoE-/-mouse’s ELAM1in aorta tissue levels were lower.In Guan Xinping high dose group, amlodipine group and atorvastatin group apoE-/mice ELAM1aortic tissue content is reduced, by comparing with the model group, a significant difference (P<0.01). These results suggest that Guan Xinping can inhibit the apoE-/-mouse aorta tissue ELAM1expression and secretion.(2) Compared with the control group,Model group apoE-/-mice aortic tissue was significantly elevated ELAM1mRNA (P<0.01).After the treatment of atorvastatin, amlodipine, low-dose Guan Xinping, high doses of Guan Xinping, apoE-/-mouse’s ICAM1mRNA in aorta tissue levels were lower.In Guan Xinping high dose group, atorvastatin group apoE-/mice ICAM1mRNA aortic tissue content is reduced, by comparing with the model group (P<0.05). There is difference between Guan Xinping low dose group and amlodipine group (P<0.01).These results suggest that Guan Xinping can inhibit the apoE-/-mouse aorta tissue ICAM1mRNA expression and secretion..3.4Guan Xinping on apoE-/-mouse3months on high fat in TNF-α、TNF-amRNA expression(1) Compared with the control group,Model group apoE-/-mice aortic tissue was significantly elevated TNF-a.(P<0.01).After the treatment of atorvastatin, amlodipine, low-dose Guan Xinping, high doses of Guan Xinping, apoE-/-mouse’s TNF-a in aorta tissue levels were lower.In Guan Xinping high dose group and Guan Xinping low dose group apoE-/mice TNF-aaortic tissue content is reduced, by comparing with the model group, a significant difference (P<0.01). These results suggest that Guan Xinping can inhibit the apoE-/-mouse aorta tissue TNF-a expression and secretion.(2) Compared with the control group,Model group apoE-/-mice aortic tissue was significantly elevated TNF-amRNA (P<0.01).After the treatment of atorvastatin, amlodipine, low-dose Guan Xinping, high doses of Guan Xinping, apoE-/-mouse’s TNF-amRNA in aorta tissue levels were decreased.; atorvastatin group apoE-/-mouse’s aortic tissue TNF-amRNA content decreased, compared with the model group were significantly ifferent; amlodipine group compared with model group have differences (P<0.01); Guan Xinping group has not been significantly changed.4.conclusionGuan Xinping can significantly improve the ApoE-/-mice relevant indicators of atherosclerotic plaques, and RNA expression of adhesion molecules, such as varying degrees of improvement, coronary vessel wall AS level can be reduced mouse TNF-a, ICAM-1, ELAM-1expression, suggesting that coronary flat with anti-inflammatory effects.