The Effect Of Shexiang Baoxin Wan On Myocardial Remodeling And Connexin43in HF Rats

Objective: In vitro,to observe the effects of SXBXW on hypertrophy of H9c2cardiomyocytes induced by Isoproterenol (Iso), including cell shape and size,cellproliferation rate and the expression of Connexin43(Cx43). In vivo,to observe theeffects of SXBXW on overload-induced HF rat model by pressure, includingmyocardial remodeling,the expression and distribution of Cx43.Methods:1）In vitro,we randomly divided the growth of good H9c2myocardialcells into normal control group, ISO group and ISO+SXBXW group. After cells werecultured48hours, we measured myocardial cell proliferation rate by MTT. After cellswere cultured72hours, we measured the myocardial cell surface size by microscopy,the protein concentration by BCA method and the expression of Cx43by WB.2）In vivo, we divided30male SD rats randomly into sham-operationgroup(sham-operation, SO group), heart failure group (heart failure,HF group)andheart failure+SXBXW group(heart failure+SXBXW,HS group),each10. Weestablished the rat model of heart failure by abdominal aortic constriction method, andthen fed them with SXBXW for12weeks.The HF group and SO group replaced withsaline. The rats after measured by cardiac ultrasound B were killed.Then take the leftventricle muscle tissue fixed with10%formalin,embedded with paraffin and cut intosliees. At last, we observed the myocardial remodeling by HE staining, thedistribution and expression of Cx43protein in the left ventricular myocardial tissue byimmunohistoehe mie-alstaining, the expression of Cx43in the left ventricularmyocardial tissue by WB.Results:1) Cardiomyocyte hypertrophy model was successfully constructedinduced by Iso,compared with the ISO group, in ISO+SXBXW group cellularsurface and protein content decreased, the rate of cell proliferation and expression ofCx43increased (P <0.05). 2) Heart failure model of rat was successfully constructed, compared with theHF group, in the HS group the left ventricular cavity diameter asignificantly reducedand EF of rats significantly increased (P <0.05),myocardial cells arranged in neat,distribution of Cx43was more regular,and expression of it increased (P <0.05).Conclusions:1) SXBXW can prevent the H9c2cardiomyocyte hypertrophyinduced by Iso and downregulation of Cx43expression.2) SXBXW can improve heart function, prevent myocardialremodeling of heart failure,and increase the expression of Cx43in myocardial tissue.