| Objective:To observe the effect of Electroacupuncture on the content of high sensitive C-reactive protein (hs-CRP) and interleukin-6(IL-6) and the expression of tumor necrosis factor-a (TNF-a) in Aortic sinus plaques in ApoE-/-mice plasma. We expect to explore the effect and possible mechanisms of EA treatment on atherosclerosis (AS).Methods:30successfully-modeled ApoE-/-mice were randomly divided into three groups as Model group, Drug-control (DC) group and Electro-acupuncture (EA) group, with10mice in each group. EA group was treated with acupuncture in Shenmen,Neiguan,Housanli and Sanyinjiao, connecting with the Hans electroacupuncture device. Meanwhile, the gavage administration of40mg/kg.d simvastatin was adopted in DC group. The coutent of hs-CRP and IL-6in mouse plasma was detected by ELISA and the expression of TNF-α was by Immunohistochemistry(IHC).Results:(1) Effects of hs-CRP and IL-6in plasma:Compared with Model-group, the content of hs-CRP and IL-6in EA group obviously decreased (P<0.01);(2) Effects of TNF-a in aortic sinus plaques:Compared with Model-group, Cells expressing TNF-a protein in an unit view of aortasinus’intima in EA group obviously decreased (P<0.01); IHC showed that there was plenty of TNF-a of modle group distributed on fibrous cap and a large scale of necrotic core and cholesterol crystals was found in aortic sinus plaques.While in EA group, there was little TNF-α on fibrous cap without significant necrotic core and cholesterol crystals.Conclusion:(1) EA can obviously decrease the content of hs-CRP and IL-6in plasma of ApoE-/-mice.(2) EA can obviously decrease the expression of TNF-α in aortic sinus plaques of ApoE-/-mice.(3) Electroacupuncture treatment can protect vessel endothelium and inhibit the development of plaques by improving the mice body’s inflammatory response state, indicating that anti-inflammatory effect may be one of the mechanisms of AS treatment with Electroacupuncture... |
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