Examination Of PCDH10Gene Methylation Status And Its Clinical Significance In Prostate Cancer

ObjectiveCurrently,the early diagnosis biomarker of prostate cancer is lack andother diagnosis biomarkers is low specificity in clinical. We detect the tissue ofprostate cancer, benign prostatic hyperplasia and prostate cancer cell linesmethylation in gene PCDH10and subsequently we collected the patient’sclinical data which corresponded to the prostate cancer samples, such asprostate specific antigen (PSA), Gleason score, clinical stage and the age ofpatients,Then to rearch whether there have some relevance between thedegree of methylation of gene PCDH10and these clinical data. So as to studythe etiology of prostate cancer and to find an early and effective biologicaldiagnostic marker for prostate cancer theoretical basis and provide anexperimental basis.Methods1、we collected the specimens of patients at the First Affiliated Hospital ofLiaoning College of Medicine and Urology, Peking University First Hospitalbetween September2012to May2013after the surgery of concurrentlaparoscopic radical prostatectomy or transurethral resection.And then savingthem in the refrigerator at-80℃.2、We selected three prostate cancer cell lines:Du145, PC3, Lncap andone normal human prostate cell lines which were cultured routinely.3、We collected benign prostatic hyperplasia and prostate cancer for DNAextraction.4、The extracted DNA was subjected to bisulfite modification. 5、PCR amplification were performed on the target DNA after bisulfitemodification.6、Collating and analysising the statistical significance between thePCDH10gene methylation and the clinical data of the patients such asprostate-specific antigen (PSA), Gleason score,clinical stage and age ofstatistical significance.Results1、In the three prostate cancer cell lines,there have two PCDH10genemethylation detected;24of40cases of prostate cancer samples were detected and thePCDH10gene methylation ratio was60.0%, PCDH10methylation of13cases ofbenign prostatic hyperplasia genes haven’t been detected.2、We compared the methylation and unmethylation of the PCDH10gene inpatients the difference in age, prostate-specific antigen (PSA) and other indicators ofclinical stage There was not statistically significant (P>0.05), but we find that foundthe methylation rate of prostate cancer samples in which the Gleason score8to10was higher than the methylation rate of the Gleason score4-7packets groupscorresponding to the samples of prostate cancer. There have statistical significancebetween the two Gleason score groups (P<0.05).Conclusions1、The methylation of PCDH10gene in prostate cancer cell lines was67.7%,the methylation rate of PCDH10gene in prostate cancer tissue samples was60%,suggesting that PCDH10gene methylation rate is higher in prostate cancer.2、Difference to the methylation situation of PCDH10in prostate cancer, thePCDH10gene methylation of13cases in the benign prostatic hyperplasia was notdetected. Significant differences between the two groups was statistically significant3、The methylation rate of Gleason score at8to10of prostate cancer sampleswas significantly higher the Gleason score at4-7groups corresponding to the samplesof prostate cancer, So we can make the following inference: the higher the higherGleason score, the higher CpG island methylation rate of PCDH10gene. Therefore we presumed the PCDH10gene methylation may play a critical and potential role in hedevelopment of prostate cancer.