The Prevalence Of Occult Hbv Infection And Molecular Evolutionary Characteristics Of OBI Virus S Gene Among The Urumqi County Adolescent Population

Objective:To understand the situation of occult HBV infection and molecular evolutionary characteristics of OBI virus S gene among the adolescent population.Methods:Getting structured questionnaires of student and their mother while collecting venous blood samples with their consent, of study, by using cluster sampling method for cross-sectional survey.Using Epidata3.1software, double double over the strategy of the original data entry and verification locked. Analysis of risk factors for HBV infection, anti-HBs negative factors and laboratory test results though SPSS15.0. For all samples using electrogenerical chemiluminescence assayed HBsAg, ELISA method detected anti-HBs, and then get amplificated of HBV S gene by nested PCR.If it was PCR (+) after nested PCR, analyze the characteristics fragment molecular evolution of adolescent population.Results:①The survey of1712, HBsAg carrier rate is1.93%(33/1712), OBI infection were a total number of103, HBV infection rate was7.94%(136/1712).Age, ethnicity, skin trauma treatment and pregnant7-9month immunoglobulin vaccination HBV infection are the major factor of affecting.②Anti-HBs negative rate was42.41%(712/1679) in1679primary and secondary students.It will be more susceptible to anti-HBs negative with nation, premature birth, the first dose of hepatitis B vaccine and hepatitis B vaccine inoculation is not timely first0-1-6full immunization vaccinated showing by logistic regression analysis.③OBI infection rate in this survey was6.13%(103/1679).Those HBsAg (+) were isolated four dominant HBV virus, HBsAg (-) samples were obtained103OBI strains.85cases of genotype B virus strains, one case of aywl serotypes than84cases were adw2serotypes;16cases of genotype C virus strains, one case of adw2serotypes than15cases were adrq+serotypes;6cases of genotype D strains, the two cases is adw2serotype I4cases ayw2serotypes. B genotype strains occurP127S, G130R and N146S mutation in the "a" determinant region, C genotype OBI strains occurred T126I and T143S mutant in the "a" determinant region, D genotype OBI strains occurred T126I, P127S, F134Y and T143S mutations in the "a" determinant region.Conclusions:①There are still quite a number of anti-HBs negative among adolescent population, hepatitis B vaccine should be timely booster vaccination;②there is a certain proportion of young people infected populations OBI, B genotype is the possible dominant genotype. OBI strains in the "a" determinant region occur T126I、P127S、 G130R、F134Y、T143S and N146S in amino acid mutations and prevent problems of OBI need to be more attention, the possible risk factors of people infected with OBI also should be a further exploring.