Research On The Comparison Of Identification Feature Of The Mixed Stasis-heat Pathogenesis Between Chronic Hepatitis B And Acute Apoplexy

ObjectiveTo explore macroscopic syndrome and microscopic indicators related to immunity of t he mixed stasis-heat symptom through clinical research of small sample of chronic hepati tis b (CHB) and acute phase of stroke (stroke) in order to preliminary establish macrosc opic syndrome representation and common microscopic indicators of the mixed stasis-heat pathogenesis related to the two diseases, further more to establish similarities and differ ences of macroscopic syndrome and microscopic immune indexes of "the mixed stasis-he at pathogenesis".Method(1)On the basis of early work,the scale of the mixed stasis-heat about CHB and strok e was developped. integration and elimination standard were determined.(2)The CHB patients were divided into two groups,the mixed stasis-heat symptom gro up(contains22patients) and the without stasis-heat symptom group(contains20patients). Meanwhile, the stroke patients were divided into two groups,the mixed stasis-heat sympto m group(contains20patients) and the without stasis-heat symptom group(contains16pati ents).The diagnosis,syndrome,physical and chemical inspection of patients should be filled in the clinical questionnaire.Fasting blood5ml samples of every patient were taken,pro cessed as requested and stored in the refrigerator of-70℃.(3)Also measurement indicators included interleukin-1(IL-1),interleukin-4(IL-4),interleuki n-6(IL-6),interleukin-8(IL-8),interleukin10(IL-10),interleukin-13(IL-13),heat shock protein-70(HSP-70),tumor necrosis factor-a(TNF-a),C-reactive protein (CRP),soluble platelet endot helial cell adhesion molecules (sPECAM-1),thrombin (TM),fibrinolytic enzyme (PLG) and platelet activating factor (PAF) should be tested.Result(1)Syndrome of the mixed stasis-heat:①CHB:the top30%signs and symptoms of t he mixed stasis-heat patients were following:yellowing of the eyes, loss of appetite, gr easy coating, yellow coating,dark tongue,trunk and muscle weakness,dry mouth,liver palm, spider angioma,soreness of limbs and trunk,orange yellow face.bitter mouth.red tongue,ab dominal distension,tongue with tooth prints;the top30%signs and symptoms of the witho ut stasis-heat patients were following:yellow urine,yellowing of the eyes,loss of appetite,t ongue with tooth prints,plump tongue,pale tongue,trunk and muscle weakness,greasy coati ng, soreness of limbs and trunk,spider angioma,liver palm,bitter mouth,yellow face,spleno megaly,ascites,obesity,white coating,distending pain in hypochondrium,poor sleep,belching. ②Stroke:the top30%signs and symptoms of the mixed stasis-heat patients were follow ing:greasy coating,dark face and lip,yellow coating,dark tongue,trunk and muscle weakne ss,dry mouth,red tongue,dysphasia,the deviation of tongue,dry stool,sublingual vein of purp le colour,purple tongue,dyschesia,bitter mouth,feverishness in palms and soles;the top30%signs and symptoms of the without stasis-heat patients were following:dark face and li p,greasy coating,dark tongue,trunk and muscle weakness,dysphasia,lassitude and somnolenc e,dry stool,plump tongue,purple tongue,loss of appetite,the deviation of tongue,pale tongue, dyschesia,dizziness,white coating,weak breath and laziness to speak.(2)Physical and chemical indicators:The level of TBIL and DBIL increased markedly (P<0.01);the level of A.HDL and LDL decreased markedly(P<0.01);the level of ALT and GLU increased significantly (P<0.05);the level of CHO decreased significantly (P<0.05);the level of HB,PLT and TG had a downward trend (0.05<P<0.1);the level of PT and APTT had a rising trend (0.05<P<0.1) between the mixed stasis-heat and th e without stasis-heat patients of CHB.But there was no visible statistical difference betwe en the mixed stasis-heat and the without stasis-heat patients of stroke.(3)Experimental study:The level of IL-1,IL-6,IL-8and HSP-70increased markedly (P <0.01), the level of IL-4,IL-13,CRP,sPECAM-1and TM elevated significantly (P<0.05);t he level of IL-10and PLG had a rising trend (0.05<P<0.1) between the mixed stasi s-heat and the without stasis-heat patients of CHB.The level of IL-1,IL-4,HSP-70,CRP an d sPECAM-1increased markedly;the level of IL-10,IL-13and TM elevated significantly (P<0.05);the level of IL-6had a rising trend (0.05<P<0.1) between the mixed stasis-heat and the without stasis-heat patients of stroke.There was markedly statistical differenc e on the level of CRP between CHB and stroke of the mixed stasis-heat (P<0.01).Mea nwhile there was no visible statistical difference on the level of IL-1,IL-4,IL-6,IL-10,IL-13,HSP-70,sPECAM-1and TM (P>0.05)Conclusion(1)Representations of syndrome between the mixed stasis-heat and the without stasis-h eat group of the same disease were obviously different.Representations of syndrome whic h were associated with the mixed stasis-heat of different diseases were lack of consistenc y.(2)The level of blood routine, biochemical examination and coagulation index were si gnificantly different between the mixed stasis-heat and the without stasis-heat patients of CHB.There was no visible statistical difference on the level of the physical and chemical indicators between the mixed stasis-heat and the without stasis-heat patients of stroke.Th us,the representations of physical and chemical indicators of different diseases were lack of consistency. Whether conventional physical and chemical inspection can be representati ons of the mixed stasis-heat pathogenesis depends on different diseases.(3)The indexs of IL-1, IL-4, IL-6, IL-10, IL-13, CRP, HSP-70, sPECAM-1and TM which rised in the mixed stasis-heat group of CHB and stroke may be common immune indexs.The level of CRP was more significant higher in the mixed stasis-heat group of stroke than CHB.CRP is closely associated with stroke.This study laid the foundation for investigating biological nature and establishing the clinical identification network of the mixed stasis-heat pathogenesis.