The Effect Of Curcumin Nanoparticles On Cell Daoy In Human Medullablastoma

Objectives: Medulloblastoma (MB) is one kind of the malignanttumors of the central nervous system in children, usually treated by surgerywith poor efficacy, high mortality and poor prognosis. It has been reportedthat curcumin (Cur) has a strong inhibitory effect on medulloblastoma cellswith nearly non-toxic side effects. However, due to its lack of solubility inaqueous solvents and poor stability, curcumin isn’t widely used in clinicaltreatment. Though the facts that curcumin nanoparticles (Cur-N) canincrease the bioavailability and reduce the medication dosage of curcuminwas confirmed by pharmaceutical study, the question if it may enhance thebiological activity against medulloblastoma is still unclear. The study aimsto compare the inhibitory effect of curcumin nanoparticles with freecurcumin on medulloblastoma Daoy cell lines and to explore whethercurcumin nanoparticles has a higher efficacy and safety than free curcumin.Method：Daoy cell lines were cultured in vitro and divided into fourgroups with different interventions: the control group (Daoy cell lineswithout any treatment), the original drug group (treatment with freecurcumin), the preparation control group (treatment wih blank nanoparticle carrier solvent), preparation group (treatment with curcuminnanoparticles).The drug concentration gradient was2.5μM/L,5.0μM/L,10.0μM/L,20.0μM/L,40.0μM/L, and the dealing points was24h,48hand72h. MTT was used to assay cell proliferation changes after treatment.Transwell assay was used to evaluate the cell migration, flow cytometry wasperformed to measure cell apoptosis，cell cycle and the concentration ofintracellular calcium ions.Result：MTT and transwell assay showed that the cell proliferationand migration were inhibited at different degrees in both the original druggroup and the preparation group. The inhibitory effect on cell proliferationwas the most obvious after the cells were treated for48h, the IC50of thegroups were37.3μM/L and5.2μM/L respectively. Compared with thecontrol group, the differences were statistically significant (P <0.05). Flowcytometry analysis showed that the original drug group and the preparationgroup had significant inhibitory effect on Daoy cells，their apoptotic ratewere higher than the control group(P <0.05) as well as the intracellularcalcium concentration (P<0.05). Meanwhile, the inhibitory effect were alsoapparent on the cell cycle which showed that more cells remained in S/G2phase (P <0.05), even the G2/M phase arrest. Moreover, all of ourexperiments demonstrated that the preparation group had stronger inhibitoryeffect than the original drug group, the difference was statistically significant(P<0.05); there wasn’t statistically significant difference of effect between the preparation control group and the control group (P>0.05).Conclusion: Curcumin nanoparticles enhanced the biological capacityof curcumin on inducing apoptosis, inhibiting proliferation and migration,and then significantly increasing the inhibitory effect of curcumin on Daoycells in medulloblastoma so that it has better anti-tumor biology activity andsafety. In summary, our study suggest that curcumin nanoparticles maybecome a substitute formulation for free curcumin and apply more widely inthe medulloblastoma clinical treatment in the future.