The Effect Of Curtn On Cholesterol Uptake In HepG2Cells

Aim: To study the effect of CurTn on the cholesterol content in HepG2cells and toexplore the molecular mechanism.Methods: HepG2cells were cultured in DEME containing10%FBS. The viability ofHepG2cells was detected by MTT. The intracellular lipid accumulation was observedby oil red O staining. The intracellular cholesterol level was detected by enzymaticassay. The mRNA expression of SREBP-2and LDLR was detected by qRT-PCR.Results: MTT assay showed that a significant decreased the cell activity of HepG2cells after treating with20μM CurTn for24h compared with the control (P<0.01)and compared with the solvent group (P<0.05), while CurTn (5μM,10μM,15μM)treated HepG2cells with24h, cell activity did not change significantly; HepG2cellswere treated with15μM CurTn or DMSO for36h, the cell activity was significantlyreduced compared with control (P<0.01). Therefore, the follow-up experiment with5μM,10μM,15μM CurTn for24h. Oil red O staining shows that5-15μM CurTnpromote cell lipid accumulation in a dose-dependent manner compared with LDLgroup. The results of enzymatic method display the concentration of TC, FC wereincreased with a dose-dependent manner after treating with5-15μM CurTn comparedwith the LDL group; while the concentration of TC, FC were increased significantlyafter treating with15μM CurTn compared with the (300μM) niacin group; butcompared with the Cur group, the content of TC was increased significantly aftertreating with15μM CurTn. The results of qRT-PCR display the mRNA expression ofSREBP-2, LDLR were decreased after treating with LDL compared with the control;the mRNA expression of SREBP-2, LDLR were increased with a dose-dependentmanner after treating with5-15μM CurTn compared with LDL group; the mRNAexpression of SREBP-2, LDLR were increased after treating with Cur compared withLDL group; the mRNA expression of LDLR was increased but the mRNA expressionof SREBP-2was not changed after treating with Ezetimibe compared with LDL group;the mRNA expression of SREBP-2, LDLR were not changed significantly after treating with niacin or DMSO compared with LDL group.Conclusion: CurTn promotes HepG2cell uptake LDL to increased cellularcholesterol levels, and the mechanism may be relate to up-regulation the mRNAexpression of SREBP-2and LDLR.