The Primily Screening Of Molecular Markers Of Bladder Urothelial Carcinoma Based On High-throughput Sequencing

Objective:To explore the occurrence and development of bladder urothelial carcinoma of the possible mechanism based on trans criptome technology from the perspective of transcription and protein expressio n, and to screen early diagnosis and monitoring of molecular markers of bladder cancer.Methods:This project adopted high-throughput sequencing technology to perform transcriptome sequencing and bioinformatics analysis on non-tumor and tumor tissue samples, screened and identified the differentially expressed genes of bladder cancer. Meanwhie we verified our hypothesis and identify the candidate targets such as CDH1,CLDN7,PTPRF and VEGFA by performing RT-qPCR on more than31cases of bladder cancer. At the same time, immunohistochemical tests for the CDH1and CLDN7were performed to explore the molecular mechanisms and clinical significance in the development of tumor.Results:1. CDH1, VEGFA, PTPRF and CLDN7may be the possible markers of early diagnosis and mornitoring for bladder cancer. The four candidate genes (CDH1, VEGFA, PTPRF and CLDN7) in tumor tissues were up-regulated compared with normal groups in samples of sequencing.2. The relative expression of VEGFA mRNA was higher than that in normal tissues. There were significant difference between the two groups (P<0.05). PTPRF and CLDN7were also up-regulated, but no significant difference was found (P>0.05).3. Stepwise logistic regression analysis were done for CDH1, VEGFA, PTPRF and CLDN7with the risk of tumor recurrence, and PTPRF was entered into the multivariate logistic regression analysis model (P=0.002), it revealed that PTPRF was closely related to tumor recurrence.4. The immunohistochemical assay of CDH1and CLDN7showed that no significant difference was found between tumor and non-tumor tissue (P>0.05), but on the contrary with CLDN7(P<0.05).Conclusion:Differentially expressed genes such as CDH1, VEGFA, PTPRF and CLDN7were incorporated into the molecular marker candidate set of the possible early diagnosis and treatment of bladder cancer. VEGFA may be the possible diagnosis markers of bladder cancer in the perspective of RNA, and PTPRF may closely related to bladder cancer recurrence.