The Clinical Features And Plexin C1、LIMK Ⅱ And Cofilin-1Plasma Level In Pulmonary Fibrosis Patients

ObjectiveThe aim of the study was to expore the cinical features between IPF and CTD-ILD and explore the relationship between plasma plexin C1、 LIMKⅡ、 CFL-1and pulmonary fibrosis through analyzing the clinical symptoms and signs, pulmonary function test, blood gas analysis, radiography, laboratory tests of the IPF and CTD-ILD patients and detecting plasma plexin C1, LIMK Ⅱ,CFL-1of them and the healthy controls.Materials and MethodsTwenty-eight patients with IPF, thirty patients with CTD-ILD, and twenty-eight healthy volunteers were enrolled to the study. The patients with pulmonary fibrosis were recruited in respiratory department of the second xiangya hospital of Central South University during the period from September2011to March2014. Healthy controls were recruited from outpatients’clinics of the second xiangya hospital of Central South University. We collected and compared the clinical symptoms and signs, pulmonary function test, blood gas analysis, radiography, laboratory tests of IPF and CTD-ILD patients to expore the cinical features of pulmonary fibrosis patients. The plasma plexin C1、LIMK Ⅱ、CFL-1level were detected in all the subjects by ELISA.We analyzed the correlations among plexin C1, LIMK Ⅱ and CFL-1and the correlation of the three indicators with lung function.Results1. The prominent clinical signs and symptoms of IPF group and CTD-ILD group were cough、dyspnea and lung Velcro sound. Compared with the blood sedimentation, C-reactive protein, PaO2and lung function indexes(FVC/Pre%、TLC/Pre%and DLCO/Pre%) of the two groups with healthy control group, there were significant differences (P<0.05), but there were no statistically significant differences between these two groups (P>0.05).2. The plasma plexin C1was (0.79±0.26) ng/ml in IPF groups and (0.78±0.21) ng/ml in CTD-ILD groups, while it was(0.39±0.08) ng/ml in normal subjects.There were significant differences between these two groups and normal subjects (P<0.05), but there were no significant differences between IPF group and CTD-ILD group (P>0.05).3. The plasma LIMK Ⅱ was (53.72±20.48) pg/ml in IPF groups and (57.02±25.83) pg/ml in CTD-ILD groups, while it was (28.77±5.81) pg/ml in normal subjects.There were significant differences between the two groups and normal subjects (P<0.05), but there was no significant difference between IPF group and CTD-ILD group(P>0.05).4. The plasma cofilin-1was (78.71±19.37) pg/ml in IPF groups and (76.93±24.55) pg/ml in CTD-ILD groups, while it was (32.18±8.52) pg/ml in normal subjects. There were significant differences between the two groups and normal subjects (P<0.05), but there was no significant difference between IPF group and CTD-ILD group(P>0.05).5. The plasma plexin C1、LIMK Ⅱ and cofilin-1in pulmonary fibrosis patients correlated positively with each other (R=0.54, R=0.56, R=0.59, all P<0.05).6. The plasma plexin C1, LIMK Ⅱ, cofilin-1of pulmonary fibrosis patients were respectively and negatively correlated with FVC/Pre%(r=-0.54, r=-0.41, r=-0.49, all P<0.05)、TLC/Pre%(r=-0.77, r=-0.45, r=-0.47, all P<0.05) and DLCO/Pre%(r=-0.65, r=-0.42, r=-0.48, all P<0.05).Conclusions1. There were no significant differences between the cilinical features(cough、dyespnea and lung Velcro sound) and examination(blood sedimentation, C-reactive protein, PaO2and lung function indexes) of IPF and CTD-ILD group. 2. The plasma plexin C1, LIMK Ⅱ and cofilin-1in pulmonary fibrosis patients elevated. Plasma plexin C1、LIMK Ⅱ and cofilin-1correlated with each other. Furthermore, plasma plexin C1、LIMK Ⅱ、 cofilin-1are negatively correlated with ventilation function and diffusion function of pulmonary fibrosis patients. All these suggest plexin C1/LIMK Ⅱ/cofilin-1pathway may be involved in the pathogenesis of pulmonary fibrosis.