Study On Separation And Analysis Of Active Components In Traditional Chinese Medicine By Molecular Imprinted-matrix Solid Phase Dispersion

Chinese medicine has a long history in our country, which is one of the advantage industries throuhout the world. The curative effect of many herbs not only withstood the tests of long-term medical practice, but also has been confirmed by modern science research institute. The active ingredients and molecular structures of some Chinese herbal medicine have been in whole or in part researched clearly. The active ingredients in Chinese medicine were complex and instability, and have low content. So the separation and analysis of active ingredients irl Chinese medicine was difficult. In,this paper, the extraction and purification of active ingredients in Chinese medicine were studied based on molecularly imprinted polymers which were prepared by different methods. We established molecularly imprinted-matrix solid-phase dispersion-high performance liquid chromatography (HPLC) hyphenated technique for analyzing active ingredients in Chinese traditional medicines. The main studies are as follows:1. The anthraquinones were selectively extracted from rhubarb samples by the way of molecularly imprinting-matrix solid-phase dispersion. In this experiment, the molecularly imprinted polymer was prepared by bulk polymerization using emodin as template molecule, methacrylic acid as functional monomer, ethylene glycol dimethacrylate as cross-linker. The isothermal adsorption, dynamics and selective adsorption experiments were investigated. In matrix solid-phase dispersion process, molecularly imprinted polymers were used as dispersant, the extraction conditions, including the ratio of molecularly imprinted polymer to sample, milling time, washing solvent and elution solvent were optimized.2. The quercetin was selectively extracted from Herba Lysimachiae by the way of molecularly imprinting-matrix solid-phase dispersion. In this experiment, the molecularly imprinted polymer was prepared by surface imprinting method using nano-silica as supporter, quercetin as template molecule, methacrylic acid as functional monomer, trimethylolpropane trimethacrylate as crosslinker. Compared with the traditional preparation method of molecularly imprinted polymer, surface imprinting method solves the problem that the template molecule is buried too deep and is not easy to remove. The isothermal adsorption, dynamics and selective adsorption experiments were also investigated in this study. In matrix solid-phase dispersion process, molecularly imprinted polymers were used as dispersant, the extraction conditions, including the ratio of molecularly imprinted polymer to sample, milling time, washing solvent and elution solvent were optimized.3. A simple method based on matrix solid-phase dispersion for selective extraction of camptothecin from camptotheca acuminata samples was developed using molecularly imprinted polymer as sorbent. The molecularly imprinted polymer was prepared by surface imprinting method using carbon nanotubes as supporter. Because carbon nanotubes have a high specific surface area and loose structure, these characteristics make the synthesis of molecularly imprinted polymer own high stability and adsorption performance. The isothermal adsorption and dynamic adsorption experiments were also carried out. At the same time, we optimized the process of the matrix solid-phase dispersion conditions:the ratio of molecularly imprinted polymer to sample, the dispersion time, washing solvent and elution solvent.4. The active ingredients were determined by HPLC. The recoveries are in the range of91.2%-102.3%, and the relative standard deviations are lower than8%which indicated the method satisfied the demands on the analysis of active ingredients in Chinese medicine. The experiment shows that the proposed method not only has high selectivity of molecularly imprinted polymer, but also has rapid separation of matrix solid-phase dispersion technology. It can be considered that this method has broad prospects in the separation and analysis of active ingredients of Chinese herbs.