The Expression And Significance Of W E E1in Renal Clear Cell Carcinoma

Objective:To investigate the expression and significance of W E E1protein in renal clear cell carcinoma (RCCC), as well as the relationship between W E E1and biology behaviors (Clinical stages, pathological grades and lymphatic metastases) of RCCC, providing evidence for diagnosis, therapy and prognosis of RCCC.Methods:Immunohistochemical analysis (SP method), real time PCR and Western blot were used to determine the W E E1expression in32RCCCs and25normal renal tissues. Then the relationships between clinical stage, pathological grade, lymphatic metastases and W E E1expressions of RCCC were analyzed. All statistical analyses were carried out with SPSS14.0software. The probability of less than5%was assumed to be statistically significant (P<0.05).Results:1.The positive rate of W E E1expression in RCCC was87.5%(28/32), compared with8%(2/25) in normal tissues. W E E1expressions in RCCC were significantly higher than those in normal renal tissues (P<0.01). The CT value of W E E1mRNA expression in the renal cell carcinoma was13.7±3.7, compared with4.5±2.0in normal tissues. There is significant difference between the two groups (P<0.01). Western Blot analysis indicates a significant higher expression of W E E1(5.2±1.5) in RCCC as compared with that (0.6±0.37) in normal tissues. In stage I, II and III-IV, the expressions of W E E1were3.1±0.7,4.7±0.9and6.7±0.9respectively. The expressions of W E E1in stage II and III-IV were1.5times and2.2times compared with those in stage I(P<0.05). The W E E1expression was also closely related with pathological grade. The W E E1expressions in well differentiated group, moderately differentiated group and poorly differentiated group were3.1±0.5,4.4±0.5, and6.4±0.9respectively. The WEE1expression in moderately and poorly differentiated group were1.75times and1.8times compared with the other one (P<0.05). The W E E1expression was4.3±1.1in patients without lymph node metastasis while6.8±0.6in those with lymph node metastasis; The W E E1expression in metastasis group was1.6times as the other group (P<0.05). There was positive correlation between expression of W E E1protein and metastasis. The correlation coefficient is0.805,(P<0.01).Conclusion:WEE1expression in RCCCs higher than those in normal renal tissues, the expression of W E E1was much lower in the normal kidney tissue. WEE1expression in RCCC was closely related to pathological grades, clinical stage and lymph node metastases. The W E E1expression in Ⅲ, Ⅳ group was higher than the phase Ⅰ and Ⅱ. The expression in the differentiation of low and moderately differentiated groups was higher than the high group. In addition, the expression of lymph node metastasis group was higher than other groups. WEE1is expected to become an important biological index to evaluate RCCC metastasis and prognosis and may be a novel therapeutic target of RCCC...