Reversal Of Resistance To Adriamycin By Compound T20in K562/ADM Cells Mediated By P-gp

Objective: To investigate the reversal of resistance to ADM by Compound T20inK562/ADM cells mediated by P-gp.Methods: Effects of K562and K562/ADM cells in compound T20on Rh123accumulationwas measured by high content; using MTT method, determination of compound T20on K562andthe cytotoxicity of K562/ADM cells, and its antineoplastic drug resistance reversal effect of ADM;To determine the effects of K562and K562/ADM cells in compound T20on ADM accumulationwith high content; Determination of the content of compound T20on the expression of P-gp onthe membrane of K562/ADM using Western Blot.Results: Compound T20could dose dependently increased Rh123accumulation inK562/ADM cells, had no significant effect on K562cells. Compound T20on K562andK562/ADM cell survival rate had no significant effect, can increase the dose related and thereversal of ADM times, but had no effect on K562cells; compound T20could significantlyincrease the accumulation of cells in K562/ADM ADM, had no significant effect on K562cells.Compound T20of the expression of P-gp at high concentration decreased significantly.Conclusions: Compound T20inhibited K562/ADM cell membrane P-gp efflux function;compound T20can increase the anticancer drug ADM on multidrug resistance cell cytotoxicity,with MDR reversal activity; compound T20can by increasing the intracellular concentration ofantineoplastic agent ADM to reverse the multi drug resistance of tumor cells; compound T20hasdrug resistance reversal activity, associated with a reduced K562/ADM cell activity and theexpression of P-glycoprotein.