| Objective:Dynamic changes of serum detected by hypertensive cerebral hemorrhage in vascular endothelial growth factor (VEGF) and tumor necrosis factor-a (TNF-α) is not the same way after the onset of expression, and to explore its involvement in the pathophysiology of cerebral hemorrhage and after its dynamic variation. By studying VEGF, TNF-a relationship with disease severity, as well as the study of VEGF and TNF-a correlation. To investigate the role of VEGF and its clinical significance HICH and TNF-a in the pathogenesis.。Methods:1Select October2011-January2014in our hospital treatment96patients with hypertensive cerebral hemorrhage patients as research subjects of the experiment, the subjects are in line with the diagnostic criteria of hypertensive intracerebral hemorrhage (with a history of hypertension, admission higher than normal blood pressure measurement), all patients have been diagnosed with brain CT scan of brain hemorrhage, and basal ganglia hemorrhage bleeding sites are not broken into the ventricle, but not for surgery patients. Exclusion criteria were:1, intracranial hemorrhage caused by trauma,2, or cerebral aneurysm ruptured intracranial vascular malformations caused by bleeding,3, stroke, brain tumors who,4, associated with acute and chronic inflammatory lesions and severe systemic dirty control diseases,5, late hernia bilateral mydriasis. In96patients,46males and50females, aged37to70years, mean (52,6±10.4) years; duration of1h-7d, average (27.52±17.32) h, according to the time of onset to admission into6groups,<5h26patients,5-15h22patients15-30h21patients,30-50h10patients,50-80h10patients,>80h7patients, respectively, at the time of admission blood once every pumping venous4ml; elect the same period in our hospital medical health examination in36cases in the control group,16males and20females; aged35to69years, mean (52.6±8.9years). Differences hypertensive cerebral hemorrhage and control groups in age structure and sex was not statistically significant (P<0.05), comparable. Normal control group also took blood4ml. Blood samples were collected after standing for30minutes, then centrifuged at3000r/min centrifuge10minutes, centrifuged to remove red blood serum collection tube below,-80℃refrigerator.2for all enrolled patients with Glasgow Coma Score (GCS), to assess the state of consciousness and neurological status of the patient. Divided into three types of light-weight, score result:Heavy GCS3-8minutes25cases, medium GCS9-11of46cases, light GCS12-14of25cases;3double antibody sandwich ELISA serum VEGF and TNF-α detection.4Statistical Methods:All data is processed according to statistical theory, using spss analysis of variance and t test. Correlation analysis using spearman rank correlation method. Inspection standards as p<0.05.。Dynamic changes of serum detected by hypertensive cerebral hemorrhage in vascular endothelial growth factor (VEGF) and tumor necrosis factor-α (TNF-α) is not the same way after the onset of expression, and to explore its involvement in the pathophysiology of cerebral hemorrhage and after its dynamic variation. By studying VEGF, TNF-a relationship with disease severity, as well as the study of VEGF and TNF-a correlation. To investigate the role of VEGF and its clinical significance HICH and TNF-a in the pathogenesisResults:(1) in the experimental group in the5-15h VEGF concentration increases at the beginning,15-30h,30-50h continues to rise to50-80h to reach peak concentration, and then decreased, but the value is still higher than between80h-7d normal, except that serum VEGF concentrations were compared with control group showed no significant difference when the disease is less than5h outside (P>0.05), the average of other groups VEGF levels were higher than normal control group (P<0.01). Among50-80h period compared with the other five numerical difference was statistically significant (P <0.01).(2) is not the same way, different levels of disease severity VEGF comparison results:the average value of VEGF heavy when <5h,5-15h,15-30h,30-50h,50-80h,>80h were significantly higher light at the average group (P<0.01), while in the group with more than80h when heavy medium group was not statistically significant (P>0.05), the average value of VEGF in medium-sized5-15h,15-30h,30-50h,50-80h,>80h were higher than the mild group (P<0.05), while<5h when comparing no statistically significant (P medium group and mild group>0.05). Tip serum VEGF concentration and the severity of a certain relationship.(3). Onset<time5h serum TNF-a began to increase, to30-50h and reached a peak and then decline,>80h was still higher than normal. In addition to the incidence of<5h group outside of serum TNF-a values of the other five patients were higher, the difference was statistically significant compared (P<0.05).。(4). GCS score differences between groups TNF-a values were statistically significant (P <0.05). The results showed that:the more severe the disease, the higher TNF-a values, so GCS score was significantly negatively correlated with TNF-a concentrations. Showed increased concentrations of TNF-α can predict HICH serious condition. duration of1h-7d, according onset to admission time is divided into six groups,<6h26cases,6-12h22cases,12-24h21cases,24-48h10cases48-72h10cases,>72h7cases,3h,12h,24h,48h,72h,7d after the onset of each blood were I, each pumping blood4ml; elect the same period in our hospital medical health examination in36cases in the control group,16males and20females; aged35to69years, mean (52.6±8.9years). HICH group and the control group showed no significant difference in the composition of gender and age (P<0.05), comparable. Normal control group also take blood. Blood samples were collected after standing at room temperature for30min,3000r/min centrifugal IOmin, serum was collected,-80℃preservation, all enrolled patients with Glasgow Coma Score (GCS), to assess the state of consciousness and neurological status of the patient. Divided into three types of light-weight, score result:Heavy GCS3-8minutes25cases, medium GCS9-11of46cases, light GCS12-14of25cases;3double antibody sandwich ELISA serum VEGF and TNF-a detection.4Statistical Methods All data are expressed as mean±standard deviation, using spss software t test, analysis of variance. Correlation analysis using spearman rank correlation method. Inspection standards as p<0.05.Conclusions:(1)in the experimental group in the5-15h VEGF concentration increases at the beginning,15-30h,30-50h continues to rise to50-80h to reach peak concentration, and then decreased, but the value is still higher than between80h-7d normal, except that serum VEGF concentrations were compared with control group showed no significant difference when the disease is less than5h outside (P>0.05), the average of other groups VEGF levels were higher than normal control group (P<0.01). Among50-80h period compared with the other five numerical difference was statistically significant (P <0.01).(2) is not the same way, different levels of disease severity VEGF comparison results:the average value of VEGF heavy when <5h,5-15h,15-30h,30-50h,50-80h,>80h were significantly higher light at the average group (P<0.01), while in the group with more than80h when heavy medium group was not statistically significant (P>0.05), the average value of VEGF in medium-sized5-15h,15-30h,30-50h,50-80h,>80h were higher than the mild group (P<0.05), while<5h when comparing no statistically significant (P medium group and mild group>0.05). Tip serum VEGF concentration and the severity of a certain relationship.(3). Onset<time5h serum TNF-a began to increase, to30-50h and reached a peak and then decline,>80h was still higher than normal. In addition to the incidence of<5h group outside of serum TNF-a values of the other five patients were higher, the difference was statistically significant compared (P<0.05).(4). GCS score differences between groups TNF-a values were statistically significant (P <0.05). The results showed that:the more severe the disease, the higher TNF-a values, so GCS score was significantly negatively correlated with TNF-a concentrations. Showed increased concentrations of TNF-a can predict HICH serious condition. |
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