Antidepressant Effect Of The Volatile Oil Of Myristica Fragrans Houtt

Objective: we observed antidepressant effect of the volatile oil of Myristica inmice, rats and investigated the mechanisms of antidepressant effect.Methods: Through tail suspension tests and forced swimming tests in mice, weobserved antidepressant effect of the volatile oil of Myristica in mice; and bymeasuring the content of neurotransmitters in the brain of the mice, we investigatedthe mechanisms of antidepressant effect. Through chronic mild stress in rats, weobserved antidepressant effect of the volatile oil of Myristica in rats; and bymeasuring the content of neurotransmitters and activity of monoamine oxidase in thebrain of the rats, we investigated the mechanisms of antidepressant effect.(1) Antidepressant experiment of the volatile oil of Myristica on mice: male mice,given different doses of the volatile oil of Myristica extract suspension by gavagingonce a day for7d, then observe the role of antidepressant on the mice through tailsuspension tests and forced swimming tests; After the behavioral experiments,take thebrain of the mice,and measure5-Hydroxy tryptamine, dopamine and noradrenalincontent in the brain by enzyme-linked immunosorbent assay;(2) Antidepressant experiment of the volatile oil of Myristica on rats:12ratswere assigned randomly into normal control group from seventy-two male rats,thelast rats were assigned into model group,positive control group and groups of differentdoses of the volatile oil of Myristica after giving21days of unpredictablestimulation;randomly given different doses of the volatile oil of Myristica extractsuspension by gavage once a day for21d, given unpredictable stimulation after1h ofgavaging;All rats were weight,horizontal and vertical activities and sugarconsumptions were tested before and after molding,and after oral administration.After the behavioral experiments,take the brain of the rats,and measure5-Hydroxytryptamine,dopamine,noradrenalin content and activity of monoamine oxidase in the brain by enzyme-linked immunosorbent assay;Results:(1) The volatile oil of Myristica showed antidepressant effect in the ailsuspension tests, no significant difference in comparison with FH. The medium dosegroup could significantly increase the content of5-HT in mice’ brain, no significantdifference compared with FH; The high and low dose group had a tendency toincrease5-HT,DA,NE levels in the brain.(2) After the Chronic mild stress,the body weight,the scores of rearing andcrossing and sweet water consumption of rats were all decresed.Comparing withmodel group after gavaging,the high dose of the volatile oil of Myristica showedantidepressant effect in the rats, no significant difference in comparison with FH. Thehigh dose group could significantly increase the content of5–HT,DA and reduce theactivity of monoamine oxidase in rats’ brain.Conclusion:(1) The volatile oil of Myristica has obvious antidepressant effect in the tailsuspension tests and forced swimming tests, its mechanism is related to increase thecontent of5-HT in mice’ brain; May be related to raise the content of DA and NE inthe brain.(2) The volatile oil of Myristica has obvious antidepressant effect in rat Chronicmild stress, its mechanism is related to increase the content of5–HT and DA in rats’brain; May be related to reduce the activity of monoamine oxidase in rats’ brain.