| ObjectiveThis paper has two purposes:First, to explore acupuncture, Jin’s three needle " on cerebral hypoxic-ischemic effects of autophagy-related proteins in the hippocampus SD rats, and its role is to improve the model rats acupuncture through autophagy pathway the damage of neuronal cells for the treatment of hypoxic-ischemic encephalopathy acupuncture looking for new experimental ideas; second is to explore the treatment in the same length of time, the time difference whether acupuncture intervention impact on the effectiveness of acupuncture, which is clinically study provides the necessary theoretical support.MethodsThis section reviews the literature study and understanding of the progress of modern medicine for the treatment of neonatal hypoxic-ischemic encephalopathy, Research TCM neonatal hypoxic-ischemic encephalopathy understanding and outlook, autophagy in hypoxic-ischemic encephalopathy three major content.Experimental study of this part includes two pilot projectsAs part of a modeling experiment, the use of delayed five minutes cesarean simulation intrauterine distress scenario, the whole preparation neonatal rat model of cerebral ischemia SD hypoxia birth weight newborn SD rats by the next day stand up experiment, the first14days of the ramp test, suspension test and open-field test, the first21days of brain tissue biopsy assessment modeling success, and according to the level of screening behavior in rats into the next experimentThe second experiment was needling part in the experiment to meet certain requirements of the model rats were divided into acupuncture group and non-acupuncture, acupuncture and acupuncture intervention, depending on the time into1-9groups, each acupuncture groups are seven days in a row acupuncture, acupuncture select "Jin three-needle" acupuncture points corresponding head, with some techniques and stimulation time, the pups were born on the first day of the rats14,21,28,35,42,49slope experiment, experimental and open-field test suspension in the first49days, all rats were sacrificed, the use of flow cytometry in the hippocampus of rats brain autophagy-related protein Beclin-1and LC3of the experimental findings.ResultsExperiment1, weightNewborn pups born after recording the first two days and the first14days of weight, found the newborn pups normal delivery model pups after birth weight difference between the first two days of the larger (P=0.00), to the first14days of body weight differences are not so obvious (P=0.148).2, the first two days turningThe first two days mean to stand comparison with the normal group, model group differences (P=0.034), there were significant differences (P=0.008) median, maximum, minimum, no statistically significant difference (P=0.265, P=0.791)3,14days behaviorSlope experimental normal group and model group there was a significant difference (P=0.000), and the open field test suspension experiments Central time frame did not find significant differences (P=0.066, P=0.209). Open-field test level score (P=0.002), vertical score (P=0.045) and grooming (P=0.009) in the normal group and model group there was a significant difference4, the first21days HE stainingNormal group:rats hippocampus neurons structural integrity, cell neatly arranged, clearly visible nucleolus, the nucleus round and full, partial and no red cytoplasmic staining.Model group:rats hippocampus neurons see significant abnormal changes in cell volume was significantly reduced compared with the normal group, irregular nucleolus, a large number of deformed cells, scattered distribution, some deformation karyopyknosis neurons stained red cytoplasm stained.Experiment IIA ramp experimentsRamp experiments each observation time point was not found significant differences,(P>0.05).2, suspension testSuspension experiment results of acupuncture group and the acupuncture group than21days have significant difference,(P<0.05). Difference in comparison with baseline on day21difference comparing the two groups have significant difference,(P<0.05).3, open-field testAlthough the open-field experiments each observation time point was not found significant differences,(P>0.05).4, autophagy-related protein Beclin-1and LC3Acupuncture group and non-acupuncture group compared ischemic lesions in the autophagy-related protein Beclin-1(46.07,46.19) and LC3(15.01,10.85) levels were high, can be found LC3protein acupuncture and non-acupuncture group more significant differences between groups. Beclin-1in the acupuncture group2, group3acupuncture, acupuncture6group, acupuncture group7,8acupuncture group and the non-significant difference between the acupuncture group showed no significant difference between the group and the non-protein LC3acupuncture group.Conclusion1, successfully established rat model of global cerebral hypoxic-ischemicThis modeling approach for the study of the mechanism of the whole brain and the different means of intervention effects on HIBD hypoxic-ischemic rat model, provides a simple operation, the survival rate of animals modeling methods. 2, the establishment of global cerebral hypoxic-ischemic rat model of evaluation methodsThe experiments established the model evaluation method to observe the hippocampus brain tissue morphology and pathology of animal behavior, including turning over the next experiment, the first14days behavior (ramp test, suspension test, open field test), day21HE staining of brain tissue3, the establishment of global cerebral hypoxic-ischemic rat model of screening methodsExperimental set up the ramp and open-field test unilateral data to mean±1.645times the standard deviation of the critical value, the experimental model screened next pups4, acupuncture can promote the expression of autophagy protein beclin-1and LC3inThis may suggest acupuncture to repair damaged neurons and autophagy mechanism intrinsically linked, not limited to the mitochondrial apoptotic pathway mediated pathway... |
PDF Full Text Request