The Association Between The Peroxisome Proliferator-activated Receptor γ2Pro12Ala Gene Polymorphism With Type2Diabetes Or Diabetic Nephropathy

Objective:To investigate the association between the peroxisome proliferator-activated receptor y2Pro12Ala gene polymorphism with type2diabetes or diabetic nephropathy,and analysis the risk factor for diabetic nephropathy.Methods:According to WHO1999criteria,248type2diabetic inpatients were selected(male134cases,female114cases,including diabetic nephropathy)from the medical ward of Endocrine during December2011to December2013.The control group was composed of healthy check-up outpatients(male65cases,female60cases),and the clinical data were collected.Genomic DNA was extracted from peripheral blood leucocytes of the participants. We screen the Pro12A1a polymorphism by means of PCR-RFLP in all subjects and identification the mutation by directly sequenced the PCR production. At last we compaired the difference of frequency in allele and genotype among groups.Results:1.The frequency of the Pro12Ala mutation was significantly lower in diabetic subjects than in non-diabetic subjects(P<0.05).2.In the diabetes group,the LDL-C and HDL-C have significantly difference among different genotypes(P<0.05).3.The frequency of the mutation was significantly lower in diabetic nephropathy group than in non-diabetic nephropathy group(P<0.05).4.The frequency of the Pro12Ala mutation did not differ significantly among the obese group (P>0.05)Conclusion:1. The peroxisome proliferator-activated receptor y2Pro12Ala gene polymorphism may be associated with type2diabetes or diabetic nephropathy.2. Allele A is the protective factor of type2diabetes or diabetic nephropathy.3. The peroxisome proliferator-activated receptor y2Pro12Ala gene polymorphism have effects on lipid metabolism in type2diabetes mellitus, but have no association with obesity.4. Total cholesterol、creatinine and systolic blood pressure are independent risk factors for diabetic nephropathy.