The Research Of Beta Blockers Induce Apoptosis To Proliferating Hemangioma

Research one Hemangioma pathology staging age distribution and curative effect observation of propranololObject:By comparing age distribution differences of each stage of hemangioma and propranolol treatment effect of infant hemangioma. To investigate the relationship between the ih pathological type and age, evaluation of oral propranolol in the treatment of infant hemangioma.Methods:1. Collecting80cases of surgically resected specimens of IH in tianjin children’s hospital between January2009and January2009. According to HE staining results to analyze the relationship between hemangioma pathology staging and the age distribution of the patients.2. Collecting121cases of infant hemangioma in tianjin children’s hospital between January2011and January2013,and48cases of male,73cases of female. Make a retrospective analysis of oral propranolol in the treatment of infantile hemangioma.Result:1.HE dyeing result shows:hemangioma proliferation period mainly distributed in (0～1year old), children fade period mainly (1～5years old), children fade to complete its mainly distributed in (5～11years old). P<0.05, the difference was statistically significant.2. Curative effect observation: all children within48hours after taking medicine are visible tumors had the varying degree is reduced, tension fell, the color becomes shallow, medication considerably change within1week. Followed up for3to5months curative effect evaluation: Ⅳ level mainly (0～1year old), children Ⅲ level mainly (1～2years old), children Ⅱ level mainly for children (2～3years old), grade I mainly for children (3～5years old). P<0.05, the difference was statistically significant.Conclusion:1.Combined with hemangioma growth of clinical and pathological features IH proliferation period is0～1year old, and then enter the complete subsidise period and recession period.2. Propranolol on proliferation phase of hemangioma curative effect is distinct, the non proliferation phase of hemangioma of the effect not beautiful.3. Propranolol has the inhibition on the proliferation phase cells, but its mechanism is unclear.4. Propranolol as beta-blockers, its inhibition of proliferation phase of the cell is through the beta1receptor or is the beta2receptor, or inhibit both beta receptor remains to be further research. Research two The effects of beta blockers on the proliferation of HUVECObject:Comparing the different influence of three β-blockers:metoprolol, propranolol, butoxamine respectively on human umbilical vein endothelial cells (HUVEC).To explore if beta blockers by block beta1receptor or beta2receptor, or both at the same time to inhibit the proliferation of vascular endothelial cells.Methods:1.To cultivate and appraisal HUVEC2.Using MTT assay to test the influence of three β-blockers:β1receptor blocker metoprolol, broad spectrum P-blocker propranolol, β2receptor blockers butoxamine on the proliferation to HUVEC. To compare the difference of inhibition rate between three groups.3.Using ELISA to test the influence of three β-blockers:β1receptor blocker metoprolol, broad spectrum β-blocker propranolol, β2receptor blockers butoxamine on VEGF and MMP-9to HUVEC. Comparing the difference of concentration of VEGF and MMP-9between three groups.Result:1.It was identified that the cultured cells were HUVEC.2.MMT showed the cell activity inhibition rate of the rapid grow of human umbilical vein endothelial cells increased significantly after treated by different three β-blockers in24hours.Comparing three groups of metoprolol, propranolol and butoxamine, the inhibition rate of propranolol group was higher than butoxamine group and the inhibition rate of butoxamine group was higher than metoprolol group.The defference have statistical significance(P<0.05).3.The ELISA showed that treated the rapid grow of human umbilical vein endothelial cells by β-blockers of metoprolol, propranolol, oxyamine in24hours.Compared with the control group, VEGF and MMP-9levels were decreased in three β-blockers groups. The concentration of VEGF and MMP-9in propranolol group decreased more significantly than butoxamine group,The concentration of VEGF and MMP-9in butoxamine group also decreased more significantly than metoprolol group,and the defference have statistical significance (P＜0.05)Conclusion:1. All of three kinds of p-blockers metoprolol, propranolol, butoxamine can inhibit the proliferation of human umbilical vein endothelial cells.2. All of three kinds of β-blockers metoprolol, propranolol, butoxamine can reduce the concentration of VEGF, MMP-9in umbilical vein endothelial cells.3.The Influence of β-blockers on vascular endothelial cells act together by the β1, β2receptor and the β2receptors stronger thanpi receptors. Research three The research of beta-blockers induce apoptosis to HUVECObject:Comparing the different influence of three β-blockers: metoprolol, propranolol, butoxamine respectively on HUVEC.To explore which beta receptor be blocked to induce the apoptosis of HUVEC. Aims to explore the mechanism of the β-blocker to the IH.Methods:1. Subcultring human umbilical vein endothelial cells and select the3-6generations cells in logarithmic growth phase to make the experiment.2. Using Flow cytometry to test the apoptosis of three β-blockers to HUVEC. To compare the difference of apoptosis between three groups.Result:1. HUVEC growth well and can be passaged24-48hours once time.2.Flow cytometry showed that the rate of apoptosis to the rapid grow HUVEC increased significantly after treated by the three β-blockers in24hours. The apoptosis was9.41%±0.53in the control group,12.44%±0.24in the metoprolol group,27.14%±0.56in the propranolol group and18.82%±0.91in the butoxamine group.Comparing three groups the apoptosis of propranolol group was higher than butoxamine group and the apoptosis of butoxamine group was higher than metoprolol group.The defference have statistical significance (P<0.05)Conclusion:1All of three kinds of P-blockers can induce apoptosis to HUVEC.2The P-blockers induce HUVEC to apoptosis act together by the β1, β2receptor and the β2receptor is stronger than β1receptor.3Three kinds of P-blockers may be suppressed both of β1, β2receptors to induce the apoptosis of endothelial cell for the treatment of IH, and β2receptor may play a more significant role.