Observed Proteomic Analysis Of Cerebrospinal Fluid Around The Spinal Cord Injury Patients And Efficacy Of Umbilical Cord Blood Stem Cells Transplantation In The Treatment

[Objectives]:The cerebrospinal fluid (CSF) proteins are analysed and the differentially expressed proteins are identified by using two-dimensional gel electrophoresis (2-DE) and mass spectrometry in umbilical cord blood stem cell (UCBSc) transplantation. To further explore the mechanisms of the umbilical cord blood stem cell transplantation for spinal cord injury (SCI) survivors.[Methods]:We recruited3thoracolumbar SCI inpatients, and they were from the Second Affiliated Hospital of Kunming Medical University of Department of Rehabilitation Medicine at June2011. All of them were chronic complete SCI and they were totally received umbilical cord blood stem cell transplantation for four times. The CSF were collected to do2-DE and mass spectrometry analysis before the first and the fourth time transplantation. We selected electromyography (EMG), activity of daily living (ADL), American Spinal Injury Association Socre (ASIA), autonomic function as our measurement methods and compared the intragroups changes after UCBSc.[Results]:1.25kinds of differential proteins were successfully identified, including17kinds of proteins up-regulation and8kinds of proteins down-regulation. Among them APOE, TTR, FGB, CFB and PEDF are closely related to apoptosis. inflammation, axonal regeneration process;2. The needle EMG significantly showed iliopsoas muscles motor unit potential (MUP) amplitude increased;3. The ASIA score were increased in some degree after the therapy, but it failed to improve the ASIA impairment scale;4. Around the therapy the ADL showed significant improvement in the performance of barthel index scores were increased greatly;5.Compared with before the patients’autonomic function were improved, mainly as posture hypotension control, smaller diurnal temperature fluctuations and the secretion of sweat functions.[Conclusions]:1. The umbilical cord blood stem cell transplantation for the treatment of SCI have a certain effect, but the detailed molecular mechanism remains to be further explored;2. Around the transplantation therapy of SCI, proteomics of the CSF changes.