Determination Of VWF And ADAMTS13in Patients With Perioperative Stroke

Objective: In this study, vWF antigen, ADAMTS13antigen and activity levels inpatients with perioperative stroke were detected so as to investigate their roles in thedevelopment of perioperative stroke.Methods: We investigated vWF and ADAMTS13in31patients withperioperative stroke and48healthy people from the General Hospital of ShenyangMilitary Region in this study. The study period was May2011to December2013.According to Adama Typing the33patients were divided into groups of lacunarinfarction, small area of infarction, and large area of infarction three subgroups. Thetwo groups were comparable in age, gender and other general information. Exclusioncriteria:(1) With acute or chronic infection history before surgery nearly two months;(2) With heart, liver or kidney dysfunction;(3) Suffering from autoimmune diseases,blood diseases and tumors;(4) With hypotension, coagulation disorders,or takinganticoagulants, hemostatic or antifibrinolytic;(5) There have been emergingcerebrovascular disease nearly two months. Peripheral blood3ml were collected,placed potassium citrate or EDTA anticoagulated tubes, then centrifuged for15minutes to2000r/min. The separated plasma was stored at-80℃spare. Byenzyme-linked immunosorbent assay (ELISA) to detect vWF antigen, ADAMTS13antigen content, application of residual collagen binding assay ADAMTS13activity. Using statistical software SPSS17.0statistical data on income. The results wereshowed in a way ofx±s, the two groups were compared using t test, P <0.05wasconsidered statistically significant.Results:1. The plasma vWF antigen levels were higher in patients with perioperative strokecompated to normal controls(P <0.05), the ADAMTS13activity and ADAMTS13antigen levels were lower in patients with perioperative stroke than in the normalcontrols (P <0.05).2. VWF antigen levels were highest in large area of infarction subgroup, and lowest inlacunar infarction subgroup. There was significant difference between the large area ofinfarction group and the lacunar infarction group (P <0.05).3. ADAMTS13antigen content was lowest in the large area of infarction group, andwas highest in the lacunar infarction group. The differences in ADAMTS13antigencontent among the three groups were significant (P <0.05). There were no significantdifferences among the three groups inADAMTS13activity levels.Conclusion:1. The vWF antigen levels in patients with perioperative stroke were higher than thenormal group, suggesting that patients with perioperative stroke were in vascularendothelial injury status, and vWF levels may be involved in the occurrence ofperioperative stroke development.2. ADAMTS13antigen content and ADAMTS13activity levels in patients withperioperative stroke patients lower than the normal control group, suggesting thatADAMTS13is related with the occurrence and development of perioperative stroke.3. Subgroup analysis showed the large area of infarction had the most significantlyincrease in vWF antigen levels and the most significantly reduce in ADAMTS13antigen, suggesting that elevated vWF increased brain tissue infarction andADAMTS13had a protective effect in perioperative stroke.4. High vWF and low ADAMTS13plasma levels may be risk factors for theperioperative stroke.