The Mechanism Of MMI-166to Promote Apoptosis In Human Pancreatic Cancer Cell Line SW1990

Objective:To investigate the mechanism of MMI-166to promote apoptosis in human pancreatic cancer cell line SW1990.Methods:Establishment of control and experimental groups, randomly, the human pancreatic can-cer xenograft model of SW1990was constructed. The control group was treated with normal saline, and experimental group was treated with MMI-166(200mg/kg/day). Apoptosis index (AI) was det-ected by deoxynucl-eotidyl transferase-mediated nick end labeling(TUNELmethod).The expression of p53, c-myc, bax,bcl-2, survivin, caspase-1and fas proteins,which are related with apoptosis, wer-e observed using immunohistochemistry in the tumor tissues,then to find the protein whose expres-sion is definitely different between the two groups. MMI-166of different concentrations (0,50,100ug/ml) were used to treat hunman pancreatic cancer SW1990cell for24h. Then the proteins exp-ression were measured by western blot.Results:AI in the control group (21.3±2.214) was lower than the experimental group (81.1±7.852), significantly. The IOD value of c-myc (7714.516±2228.915) and survivin (4594.058±1239.530) proteins of MMI-166group was significantly different from the control group, with a significant dif-ference(P＜0.01). Twenty-four hours after MMI-166treatment of different concentrations (0,50,100μg/ml), the c-myc expression was (0.169±0.007)、(0.098±0.003) and (0.073±0.008), with a significant difference(P<0.05); the survivin expression was (0.391±0.006),(0.395±0.006),(0.382±0.004), without a significant difference(P=0.069).Conclusion:MMI-166can induce cell apoptosis in vitro of SW1990by down-regulating the expre-ssion of c-myc.