Expression And Clinical Significance Of SET Domain Containing1α (SETD1A) In Laryngeal Squamous Cell Carcinoma

Object:The study detected the mRNA, protein expression of SETD1A and histone H3K4trimethylation (H3K4me3) in laryngeal squamous cell carcinoma and adjacent tissues to analyze the relationship between protein expression and clinicopathological parameters and the correlation between the H3K4trimethylation and SETD1A mRNA levels, explored the expression and clinical significance of SETD1A in laryngeal squamous cell carcinoma.Methods:1. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to detect differences of SETD1A mRNA expression levels between laryngeal squamous cell carcinoma tissues and adjacent tissues in paired samples43cases.2. SETD1A protein expression was detected by immunohistochemistry (IHC) in135laryngeal squamous cell carcinoma cases to statistically analyze the relationship between SETD1A protein expression and clinicopathological parameters via chi-square test.3. Proteins were extracted from43paired samples and histone H3K4trimethylation levels were assessed, and analyze the correlation between the H3K4trimethylation and mRNA levels via Pearson correlation analysis.Results:1. qRT-PCR results showed that the relative mRNA expression level of SETD1A in laryngeal squamous cell carcinoma group and adjacent tissues group (x±SD) were0.668±0.131and0.519±0.136, showed significant differences between the two groups (P<0.001). The relative mRNA expression level of SETD1A in laryngeal squamous cell carcinoma group was higher than that of adjacent tissues groups;2. IHC results indicated that SETD1A protein in laryngeal squamous cell carcinoma was upregulated. There are128positive expression cases in135laryngeal squamous cell carcinoma cases, which include in69low expression cases (51.1%),66high expression cases (48.9%). SETD1A protein in adjacent tissues group and polyp of vocal cord group were negative expression.3. Statistical analysis of clinical parameters of patients with laryngeal squamous cell carcinomas found that SETD1A protein expression is associated with primary tumor site (P=0.006), T classification (P<0.001), N classification (P=0.009), clinical stage (P<0.001) and grade of differentiation (P=0.006), but showed no statistically correlation with age, smoking history, alcohol consumption (P>0.05).4. H3K4trimethylation levels of laryngeal squamous cell carcinoma group and adjacent tissues group (x±SD) were respectively1.494±0.278(%) and0.29±0.077(%), showed significant differences between the two groups (P<0.001); H3K4trimethylation levels of laryngeal squamous cell carcinoma group was significantly higher than that of adjacent tissues groups.5. Compared to adjacent tissues, H3K4me3levels in aryngeal squamous cell carcinoma positively correlated (r=0.605) with SETD1A mRNA relative levels, and the linear regression relationship was statistically significant (regression coefficient hypothesis test P<0.001).Conclusion:1.mRNA, protein expression of SETD1A and histone H3K4me3in laryngeal squamous cell carcinoma was significantly higher than those in adjacent tissues.2. SETD1A protein expression in patients with squamous cell carcinoma is associated with the primary tumor site, T classification, N classification, clinical stages and grade of differentiation, suggesting SETD1A may play an important role in occurrence and development of laryngeal squamous cell carcinoma.3. The correlation between histone H3K4trimethylation levels and SETD1A mRNA levels is positive.