Studies On The Bioactive Constituents From Holothuria Atra Jaeger

This dissertation determined the active components of the Holothuria atra Jaeger, by MTT method, flow cytometry and fluorescent staining tracing; The extractive of H. atra Jaeger, which from south China sea were extracted and purified with the means of macroporous resin chromatography, normal phase silica gel chromatography, reversed-phase silica gel chromatography, dextran gel chromatography and high performance liquid chromatography (HPLC) and other modern chromatography separation technology.13get triterpenoid saponins monomer compounds were obtained. At the same time, the chemical structure of8compounds were measured with many kinds of The modern spectrum technology, and the eight compounds were first isolated from the H.atra Jaeger.In this paper, antineoplastic effct of the separation of the components were determined by MTT, flow cytometry and fluorescent staining methods. The results showed that the extraction and separation of the Holothuria atra Jaeger have significant anti-tumor activity, using the human lung cancer cells A549and leukemia cell lines HBL60the chemical structure of8compounds were measured with many kinds of The modern spectrum technology, including1HNMR,13CNMR、DEPT、TOCSY、COSY、HMBC、HSQC and so on, to determine the chemical structure of the eight compounds, as3-O[3""-O-β-D-methoxyl-glucopyranosyl-(1→3)-β-D-glucopyranosyl-(1→4)-β-D-quinovopyranosyl-(1→2)-4’-sodiumsulfate-β-D-xylopyranosyl]-holosta-9-ene-22,25-oxido-3,12,17-triol (Compound1, holothurin A);3-0-[3""-O-β-D-methoxyl-glucopyranosyl-(1→3)-(3-D-glucopyranosyl-(1→4)-β-D-quinovopyranosyl-(1→2)-4’-sodiumsulfate-β-D-xylopyranosyl]-holosta-9-ene-3,12,17,22-tetrol(Compound2, holothurin A1);3-O-[3""-O-β-D-methoxyl-glucopyranosyl-(1→3)-β-D-glucopyranosyl-(1→4)-β-D-quinovopyranosyl-(1→2)-4’-sodiumsulfate-β-D-xylopyranosyl]-holosta-9-ene-3,12,17,24-tetrol(Compound3, holothurin A3);3-O-[3""-O-β-D-methoxyl-glucopyranosyl-(1→3)-β-D-glucopyranosyl-(1→4)-β-D-quinovopyranosyl-(1→2)-4’-sodiumsulfate-β-D-xylopyranosyl]-holosta-9-ene-3,12,17,25-tetrol(Compound4);3-0-[3""-O-β-D-methoxyl-glucopyranosyl-(1→3)-β-D-glucopyranosyl-(1→4)-β-D-quinovopyranosyl-(1→2)-4’-sodiumsulfate-β-D-xylopyranosyl]-holosta-9-ene-3,12,17-triol(Compound5, echinoside A);3-0- [3""-O-(3-D-methoxyl-glucopyranosyl-(1→3)-β-D-glucopyranosyl-(1→4)-β-D-quinovopyranosyl-(1→2)-4’-sodiumsulfate-β-D-xylopyranosyl]-holosta-9-ene-22-one-3,12,17,25-tetrol (Compound6, holothurin A3);3-O-[3""-O-β-D-methoxyl-glucopyranosyl-(1→3)-β-D-glucopyranosyl-(1→4)-β-D-quinovopyranosyl-(1→2)-4’-sodiumsulfate-β-D-xylopyranosyl]-holosta-9,25-diene-22-one-3,12,17-triol(Compound7);3-0-[3""-O-β-D-methoxyl-glucopyranosyl-(1→3)-β-D-glucopyranosyl-(1→4)-β-D-quinovopyranosyl-(1→2)-4’-sodiumsulfate-β-D-xylopyranosyl]-holosta-9,25-diene-3,12,17-triol(Compound8, scabraside A).This research made a systematic research on the triterpenoid saponins compounds from Holothuria atra Jaeger and extracted triterpene saponin with antitumor activity compounds from Holothuria atra Jaeger for the first time. This study provided lead compounds for the research of new antitumor compounds, and theoretical basis for the development of sea cucumber medicinal value. It has very important practical significance on the development and utilization of Marine biological resources in China. This study enriched the research of Marine pharmacology and Natural Pharmaceutical Chemistry and provided some theoretical support for the development of Marine biological activity and pharmacological action of our country in the future.