Liensinine Suppresses Human Bladder Cancer Cells T24Proliferation In Vitro

Objectives：To study the effects of Liensinine on Poliferation and cell cycleof human bladder cancer T24cells. Methods：T24cells were treated withdifferent concentrations of Liensinine. Cell proliferation were detected byCCK-8and clonogenic assays. The cell cycle analysis was examined by flowcytometry. p21mRNA expression were determined by Real-time QuantitativePCR.Results：Compared with the control, Liensinine inhibited the proliferationof T24cells in a dose-dependent manner. The differences of inhibitory rates（1.5625、3.125、6.25、12.5、25μg/mL）via control groups were obvious(P<0.05). Proliferation inhibition is more obvious when interventsubcutaneously transplanted tumor by different concentration of Liensinine. Andconcentration dependent effect relationship is obvious (P<0.05). T24cellinhibition rates were （-0.5±24.5）%、（26.51±14.28）%、（43.26±5）%、（42.93±8.51）%、（55.11±3.01）%.With the concentration of methanol as acontrol group，Liensinine concentration in1.5625μg/mL are not significantlydifferentstically significant. Liensinine groups in3.125,6.25,12.5,25μg/mLrelative to methanol control groups showed significant differences, andstatistically significant (P<0.05). The inhibitory effect reached its maximum at 48h post-treatment, s-phase cells of bladder cancer T24had a significantincrease and share of possession were （23.11±0.57）%、（24.91±1.10）%、（32.10±5.18）%、（53.09±3.91）%、（55.97±0.45）%. T24cells were arrestedat the S phase. Compared with the control group, the expression of p21onmRNA level was improved significantly in the Liensinine groups(P＜0.05).Westblot results show that P21protein expression and phosphorylation of p53levels increase.Conclusions： Liensinine inhibits T24Bladder cancer cellproliferation and arrests cells at S phase，and the mechanism may have a closerelationship with the upregulation of P21and phosphorylation of p53expression.