Extraction Of Rutin. From Flos Sophorae By Aqueous Two-Phase System Consisted Of Aqueous Ammonium Sulfate-Acetone

Flos Sophorae is the flower bud of Chinese scholar tree, which is a member of the bean family. Chinese scholar tree is widely populated in the northern area of Guangxi. Traditionally it has been used to handle hematochezia, hemorrhoid, bloody flux, bleeding, hepatitis, eye infection, headache and dizziness. In clinical practice it prevents cerebral hemorrhage, hypertension, retinal hemorrhage, purpura and acute hemorrhagic nephritis.Main bioactive component in Flos sophorae is ruti. Some can reach the level of40%and above.The subject of this research is separation of rutin from Flos Sophorae by aqueous two-phase liquid extraction. Material of Flos Sophorae was extracted by water to give the extract. This extract were distributed in an aqueous liquid two-phase system which consisted of water, acetone and ammonium sulfate. Effects of distribution system on separation of rutin from extract were investigated. Orthogonal Design Method was used to optimize distribution systems. The results showed that extract from Flos Sophorae distributed in aqueous two-phase system consisted of aqueous ammonium sulfate-acetone solution ((NH4)2SO419%, acetone35%, w%, pH9.0,) gave rutin in purity of95.92%in light phase solution.