Antagonistic Effect Of Yishenhuayu Recipe Screening Effective Parts Of Renal Interstitial Fibrosis And Renal Tissues Of UUO Rat Regulation Of TGF-β1and BMP-7

Objective：Kidney Huayu Recipe (YiShenHuaYu Recipe, YSHYR) is a empiricalrecipe summed up by our mentor Professor Zhang Linqi from many years of clinicalexperience in preventing and treating renal interstitial fibrosis;Chronic renal failuresyndromes conducted preliminary studies on syndromes suggest that,"Kidney Blood"for the RIF main characteristics of the pathogenesis, the clinical application of kidneycirculation side effects certainly for many years, pre-preliminary animal experimentshave shown to be related to the regulation of cytokine expression by havingantagonistic RIF role.This experiment according to the indicator of antagonisticanti-kidney between mass fiber, through establishing big rat (UUO) model of RenalInterstitial Fibrosis, observating the effects of YiShenHuaYu Recipe on UUO big ratkidney features,kidney organization pathology injury, kidney organization in thehydroxyl breast ammonia acid content and renal interstitial fibrosis of logo proteinFN、 TGF-β1and BMP-7expression, to filter out effective parts of YiShenHuaYuRecipe antagonistic anti-kidney between mass fiber, to discussion the rolemechanism, for further researching effective parts of Chinese traditional medicinecompound by YiShenHuaYu Recipe in antagonistic anti-kidney between mass fiber inlaying the theoretical foundations of effective components.Methods:1. YiShenHuaYu Recipe in different parts of the polar extraction: weextracted four polar areas (petroleum ether, ethyl acetate, n-butyl alcohol,water-saturated)by YiShenHuaYu Recipe of Polar from smallest to largest, accordingto the method of system solvent extraction.2. Using single side ureteral ligation method to establish big rat (UUO) model ofrenal interstitial fibrosis147male SD rats were randomly divided into fake surgery groups, model groups,parts of the methods of YiShenHuaYu Recipe of petroleum ether groups, parts of themethods of YiShenHuaYu Recipe of ethyl acetate groups, parts of the founder ofYiShenHuaYu Recipe butanol group, valsartan group, each with21. All the groups areused single side ureteral ligation law to establish big rat (UUO) model of renalinterstitial fibrosis except fake surgery group; At the3,7,14d of surgery, werandomly select7rats only from each group, to detect kidney features by abdominalactive pulse blood; to observate kidney organization pathology morphology changesof SD big rats by light mirror; to detect the hydroxyl breast ammonia acid of content of kidney organization using alkaline water solution; to detect the FN of expressionchanges of kidney organization by ELISA law; to detect TGF-β1and the BMP-7of expression changes of kidney organization by immune organization law.Results：(1) YiShenHuaYu Recipe in different parts of the polar effect onkidney function of UUO model ratsCompared to Sham-operation group，model group of those gradually elevated astime extending; and compared to model group, the drug-intervention group of those atthe time points obviously reduced.(2) YiShenHuaYu Recipe in different parts of the polar effect on kidneypathological changes of UUO model ratsFake surgery group’s big rats in different time points within kidney organizationpathology have no obviously exception; model group’s big rats of kidney organizationpathology changes obviously, and as time extended gradually aggravated, mainlyexpresse for kidney small tube epithelial cell shedding, and degeneration, kidneybetween mass inflammation cell infiltration and the fiber; drug-intervention group bigrat kidney organization pathology changes in the time points are obviously light tomodel group, which to YiShenHuaYu Recipe founder butanol parts lesions most light.(3) YiShenHuaYu Recipe in different parts of the polar effect on kidneyhydroxyproline content of UUO model ratsCompared to Sham-operation group，Model groups in kidney hydroxyprolinecontent increased significantly, and as time extended gradually increased. Comparedto the model groups, drug-intervention group of hydroxyproline decreasedsignificantly, which to YiShenHuaYu Recipe founder butanol parts group andvalsartan Sutton Group more obviously, and no statistically significant differencebetween two groups (P>0.05).(4) YiShenHuaYu Recipe in different parts of the polar effect on expression ofFN in the kidney of UUO model ratsFake surgery group’s FN throughout the course of experiment has no obviouschange. Model group’s FN in Rat Renal tissue significantly increased, and as timeextended gradually increased. Compared to the model group, drug-intervention groupof FN reduced significantly.(5) YiShenHuaYu Recipe in different parts of the polar effect on expression ofTGF-β, BMP-7of UUO model ratsFake surgery group’s kidney organization in the TGF-β1positive expression veryless; Model group’s TGF-β1in Rat Renal tissue significantly increased, and as timeextended gradually increased; drug-intervention group has TGF-β1of positiveexpression in different time points obviously less than model group, YiShenHuaYuRecipe founder butanol parts group and valsartan Sutton Group more obviously, andno statistically significant difference between two groups (p﹥0.05), BMP-7isopposite.Conclusion：1.Solvent extraction method using the system successfully getYiShenHuaYu Recipe petroleum ether, ethyl acetate, butanol and saturated with waterparty site.between4apolar parts, YiShenHuaYu Recipe founder butanol parts groupobviously improved the kidney features, reduced kidney pathology injury, reduced hydroxyproline content, its other effects are similar to valsartan Sutton, and remaining3parts on polar improved no obviously. So, YiShenHuaYu Recipe founder butanolparts group might be effective parts of antagonistic effect in renal interstitial fibrosis.Founder butanol fraction of YiShenHuaYu Recipe group has a good delay betweenrenal interstitial fibrosis2. butanol fraction of YiShenHuaYu Recipe group can reduced kidney renaltissue FN levels. Its mechanism related to inhibiting expression of TGF-β1andup-regulating expression of BMP-7, indicating that it can reduce the deposition ofECM,reduce the degree of renal interstitial fibrosis with antagonistic renal interstitialfibrosis.3. butanol fraction of YiShenHuaYu Recipe group might be effective parts ofantagonistic effect in renal interstitial fibrosis.