Hypoglycemic Effects And Mechanism Of Qiangyi Jiangtang Capsules In KKAy Mice

Objective：To investigate the effects and its underlying mechanism of Qiangyi JiangtangCapsules on glucose metabolism in KKAy mice with type2diabetes.Methods：The male KKAy mice were fed with high fat-diet to induce the type2diabeticmice. The mice with the fasting blood glucose over7.8mmol/L and the decrease ofinsulin sensitivity were considered as type2diabetic mice. Fifty KKAy diabetic micewere randomly divided into diabetic model group, metformin group, low dose QJCgroup, medium dose QJC group, high dose QJC group and C57BL/6J mice served asa normal group. Mice in the QJC groups received intragastrically QJC at a dose of0.6,0.9,1.35mg/kg·d, respectively, as well as in the metformin group receivedintragastrically metformin (0.25g/kg·d). All of substances dissolved in0.5%CMC-Na were administrated intragastrically to mice once daily for nine weeks. Bloodglucose, total cholesterol and total triglyceride were detected every week. During theexperiment, sucrose and glucose tolerance and plasma insulin were measured. At theend of experiment, all the mice were killed, and the pancreas and kidney were takenout. The kidney and the part of pancreas were doused in10%formaldehyde solutionfor pathological determination. Tthe other of pancreas were stored at-80℃forassaying the contents of superoxide dismutase（SOD）, malondialdenhyde（MDA）and tumor necrosis factor (TNF-α).Results：1. Compared with model group, the random blood glucose in the QJC groups(0.6g/kg,0.9g/kg) were lowered (P＜0.01and P＜0.05) after two-weekadministration. Over the next few weeks, the random blood glucose of theQJC groups showed certain lowered, but the differences were not statisticallysignificant. 2. Compared with normal group, the fasting serum insulin of the model groupwere raised (P＜0.05), the HOME-IR were increased (P＜0.001). Comparedwith model group, the fasting serum insulin of the medium dose QJC groupand the high dose QJC group were droped (P＜0.01), in a dose dependentmanner; the HOME-IR were dreased (P＜0.05).3. At week2of adminstration, the sugar tolerance experiment showed that theblood glucose was lowered in the QJC groups at2h glucose loaded,compared with glucose unloaded. At week9of administration, comparedwith model group, the glucose tolerance of the metformin group and the QJCgroups have little increase of blood glucose (P＜0.05), the differences ofblood glucose at30min、60min and120min were small (P＜0.05).Compared with model group, the area under blood glucose concentrationcurve of the high dose QJC group were tiny (P＜0.05).4. Compared with model group, the serum triglyceride levels of the low doseQJC group lowered, have statistically significant at the week of2and7(P＜0.05); after2weeks, the serum triglyceride levels of the medium dose QJCgroup lowered, have statistically significant at the week of6and7(P＜0.01);after3weeks, the serum triglyceride levels of the high dose QJC grouplowered, has statistically significant at the week of7(P＜0.01). Its effects onserum total cholesterol levels were not statistically significant.5. The results of SOD、MDA and TNF-αin tissue homogenate of pancreas：compared with normal group, the levels of SOD of the model group weredroped, the levels of MDA were raised. Compared with model group, thelevels of SOD of QJC groups were raised, the levels of MDA and TNF-αwere droped.All these changes were not statistically significant.6. The effects of QJC on histopathology of pancreas: the model group hasobvious congestion, islet cells widely spaced and necrosis compared thenormal group, all the pathology of the dose QJC groups were better than themodel group.7. The effects of QJC on histopathology of kidney: the model group hasobvious tubular atrophy and regeneration, mesangium thickness andlymphocytic infiltration compared the normal group, all the pathology of the dose QJC groups were better than the model group.ConcLusions：1. QJC could decrease the blood glucose in the KKAy mice, its mechanism wascorrelated to promoting the utilization of glucose.2. QJC could improve glucose tolerance and insulin resistance in KKAy mice.3. QJC could improve serum triglyceride level in the KKAy mice.4. QJC could protect pancreas and kidney, which might be related to its clearingfree radical, ameliorating hyperglycemia, hyperinsulinmia and insulinresistance.