Preparation And Evaluation Of The Performance Of Molecularly Imprinted Materials Are Assisted With Crowding Reagent

Molecular imprinting is a new technique for synthesizing material of molecular recongnition with the ability of pre-selectivity. Molecular crowded technology is a kind of new way to promote effectively the molecular imprinting effect. Applying molecular crowding reagent to MIPs of drug delivery systems has longer-term research prospects. Molecular imprinting technology in the field of monolithic column is also very research potential, especially in the area of synthetic materials need to constantly develop new functional monomer, so as to meet the needs of specific structure template MIPs synthesis.The first part of this work, methacrylic acid (MAA) as functional monomer, ethylene glycol dimethacrylate (EDMA) as cross linker, polystyrene as molecular crowding agent, imprinted polymer was synthesized by precipitation with aminoglutethimide as template. The resulting MIPs were used as controlled release materials. The in vitro release study showed that:compared with not join polystyrene, polystyrene significantly enhanced the special recognition performance of MIPs, and has obvious sustained release effect. The result shows that, there is an obvious molecular crowding effect in aminoglutethimide-methyl acrylic acid system. Through equilbrium adsorption experiment, the adsorption properties of MIPs between with PS and without PS, shown that crowding reagent can stronger adsorption properties. Release model is adopted for the release of curve fitting comparison and study its release mechanism. Zero-model is the best fitting model, while without PS system is not zero order release. The results showed that the molecular crowded reagent enhance the imprinting effect of the polymer, and releasing mechanism for the zero order release.The second part of this article, a new functional monomer, ionic liquid (1vinyl-3-butylimidazole chloride salt) is the first used. In this work, under the condition of polarity porogen, with ionic liquid as functional monomer, ethylene glycol dimethacrylate (EDMA) as cross linker, methanol, isooctane (ISO) and polystyrene/tetrahydrofuran (PS/THF) as porogen, an imprinted monolith was synthesized by an in-situ polymerization with ketoprofen as template. Overall study of the preparation conditions, such as template molecule and functional monomer proportion, the proportion of functional monomers and crosslinking agent, polystyrene in tetrahydrofuran content and molecular weight of polystyrene and kinds of crowded reagent affect of column imprinting factor, combined with scanning electron microscopy (SEM), mercury intrusion and nitrogen adsorption morphology characterization and high performance liquid chromatography (HPLC) experimental conditions, further proof that the molecularly imprinted monolithic column effect. Template and functional monomer and crosslinking agent of1/4/20, molecular weight of350,000PS in THF content is40mg/mL, the imprinting factor of the column is the largest, up to2.297. Results show that ionic liquid1-vinyl-3-butylimidazole chloride salt can be used as a new type of functional monomer, under the condition of the polar porogen methanol, synthesis has the imprinting effect of molecularly imprinted monolithic column.