Based On TGF-β/Smads Signal Transduction Pathway Of Penyan Enema In The Treatment Of Sequelae Of Pelvic Inflammatory Disease Of Chronic Pelvic Pain Analgesic Mechanism

Objective:Based onintracellular TGF-β/Smads signal transduction pathway of Penyan enema in the treatment of sequelae of pelvic inflammatory disease of chronic pelvic pain pain mechanism, provide a scientific basis for the widespread clinical application for enema of pelvic inflammation.Methods:The cultured human hypertrophic scar fibroblasts, subculture, with Chinese traditional medicine Penyan enema maximum nontoxic concentrations of6.25mg/ml on cell function in human hypertrophic scar fibroblasts, with TGF-β1antibody group, TGF-β1antibody+Penyan enema group, pelvic inflammation with intestinal side group as the experimental group, were added into the culture medium group as control group, the content of Smad3and Smad7protein in supernatant was detected by double antibody sandwich ABC-ELISA method in four groups of cells, RT-PCR was used to detect the four group of cells in the TGF-β1, Smad.3, Smad7and type I collagen mRNA expression.Results:1. compared with control group, the content of Smad3three in experimental group were significantly decreased, Smad3content in the Penyan enema group, pelvic inflammation enema+TGF-β antibody group, TGF-β1antibody group were796.60±180.32pg/ml,1659.92±303.12pg/ml.2090.88±120.67pg/ml, compared with control group was2569.82±111.89pg/ml, the differences were statistically significant (P<0.05), the difference between each experimental group had statistical significance (P<0.05). Content of Smad7in the experimental group in the three group compared with the control group were significantly increased, Smad7content in the Penyan enema group, pelvic inflammation enema+TGF-β1antibody group, TGF-β1antibody group were68.91±12.62pg/ml.30.62±6.35pg/ml,14.39±1.99pg/ml, compared with the control group was0.97±1.09pg/ml, the differences were statistically significant (P<0.05). the difference between each experimental group had statistical significance (P<0.05).2. Three groups of TGF-β1, Smad3and type Ⅰ collagen mRNA expression compared with the control group decreased, gray and gray ratio in which the internal TGF-β1mRNA in pelvic inflammation enema group, pelvic inflammation enema+TGF-β1antibody group, TGF-β1antibody group were 0.44±0.03,0.91±0.23.1.46±0.26,2.19±0.04compared with the control group, the differences were statistically significant (P<0.05), the difference between each experimental group had statistical significance (P<0.05). Gray and reference intensity ratios Smad3mRNA in pelvic inflammation enema group, pelvic inflammation enema+TGF-β1antibody group, TGF-β1antibody group were0.54±0.10,1.39±0.10,1.76±0.05,2.07±0.14compared with the control group, the differences were statistically significant (P<0.05), experimental group comparison the differences were statistically significant (P<0.05). Gray and reference intensity ratios of type I collagen mRNA in pelvic inflammation enema group, pelvic inflammation enema+TGF-β1antibody group, TGF-β1antibody group were0.21±0.02,0.79±0.06,0.87±0.02,1.02±0.06compared with the control group, the differences were statistically significant (P<0.05), experimental group the comparison among groups was statistically significant difference (P<0.05). While the experimental group Smad7mRNA and control group in the three group were increased compared with control, gray gray ratio of Smad7mRNA in pelvic inflammation enema group, pelvic inflammation enema+TGF-β1antibody group, TGF-β1antibody group were1.23±0.06,1±0.15,0.76±0.06.0.57±0.05compared with the control group, the differences were statistically significant (P<0.05), the difference between each experimental group had statistical significance (P<0.05).Conclusion:Basin inflammation enema in the treatment of sequelae of pelvic inflammatory disease of chronic pelvic pain is mediated by TGF-β/Smads signal transduction pathway, increased expression of TGF-β1and Smad3pathway in reducing the negative regulator of the pathway of Smad7to reduce the gene expression of type I collagen, fibrosis degree to reduce the adhesion chronic inflammation, and pain relieving effect.