The Supernatant Of LO-2Cells To Induce Human Mononuclear Cells Transformation The Cells Like Hepatocyte And The Preliminary Study Of Cells Like Hepatocyte Infected HBV In Vitro

ObjectivesTo explore culture in vitro human mononuclear cells transformation to thehepatocyte. The cell like hepatocyte infected HBV as new cell model.Methods1The supernatant of LO-2cells to induce the isolated of humanmononuclear cells, continuous detection of AFP10days, when thepassaged cells have no inducer continuous detection of ALB10days.2The human serum of infected HBV infect Cells Like Hepatocyte andMonitor HBsAg, HBeAg expression in10days.Results1Through positive results of ALB protein tests Shows that humanmononuclear cells can transform The Cells like Hepatocyte successfully.2After The human serum of infected HBV infect Cells like Hepatocyte thepositive results of HBsAg and HBeAg protein tests.ConclusionsWe can induce differentiation the Cells like Hepatocyte successfully as thecell of infected HBV foster in vitro which lay the foundation for researchingof HBV infection and Pharmaceutical effect.