| [Objective]1, To set up the SD rats, Japanese big ear rabbits IVC ligation/Stenosis DVT animal model; To determine the DVT formation before, peak, fade of state corresponding to the key point in time; To determine the advantages and disadvantages of two methods of building and SD rats, Japanese big ear rabbit in the preparation of the advantages and disadvantages of DVT model; The next step research provides data base.2, After successful model, quantitative detection by ELISA method GP1b-α protein expressed in DVT model changes, and discussed the molecular express the meaning of the changes in the formation of DVT.[Materials and Methods]The part one IVC ligation/Stenosis model in rat, rabbit of DVT in the establishment and study1,220SD rats, female, weight for190±20g, it were randomly divided into:normal control group10, the control group10, the inferior vena cava ligation group100, the inferior vena cava stenosis group100;140Japanese big ear rabbit, female, was2.20±0.35kg weight, it were randomly divided into:normal control group10, the control group10, the inferior vena cava ligation group100, the inferior vena cava stenosis group100;2, Two kinds of animals don’t do any operation with normal control group; After exposure of the inferior vena cava in the abdomen with the control group; Inferior vena cava ligation group and inferior vena cava stenosis group respectively under vena cava ligation method and inferior vena cava stenosis method to establish animal model of SD rat DVT and Japanese big ear rabbit animal model of DVT; Record before/after inferior vena cava ligation of diameter, building time, and so on and so forth; 3, After the mould of2h,4h,6h,8h,12h,24h,3d,7d,14d and21d, each time on site, SD Rats of normal control group and the control corresponding to each executed1, inferior vena cava ligation and stenosis group corresponding to each executed10. Japanese big ear rabbit normal control group and control group corresponding to each executed1, inferior vena cava ligation and stenosis group corresponding to each executed6. Observing animals bilateral hind limbs swelling, and so on; Observe building period of inferior vena cava swelling degree, color, local tissue reaction, etc.; Measure out the inferior vena cava diameter; Collection and inferior vena cava blood5ml; Get blood clots and corresponding vein wall tissue; Measure the thrombus length; Weighing the thrombus and the total wet weight of corresponding vein wall;4, Acquisition of inferior vena cava blood anticoagulation, centrifugal separation of plasma,-80℃keep spare; Obtain and corresponding vein thrombus biopsy wall tissue and HE staining, and then based on micro situation at different time points;5, Statistics processing:using SPSS17.0software, statistical analysis of the measured data of all, the measurement data with mean+/-standard deviation (+/-S) said, the t test analysis between the two sets of data, each group with single factor analysis of variance (One-Way ANOVA), P<0.05was statistically significant.The part two GPlb-α in SD rat model of DVT expression changes in plasma1, According to the first part of the experimental results and the experimental acquisition of plasma, kept centrifugal selection of SD rats normal control group plasma10, control group of plasma10, and2h after inferior vena cava stenosis group plasma10,24h plasma10,8h plasma10,7d plasma10, using ELISA method, measuring between groups in SD rats plasma GPlb-α expression changes.2, Using SPSS17.0software, value of statistical analysis to measure the expression of change. Measurement data with mean plus or minus standard deviation (+/-S) said, using the single factor analysis of variance between groups, P<0.05, with statistical significance.[results]1, SD rats, Japanese big ear rabbit after building, building period of inferior vena cava came first black clot material inside, and blended in black and white clot material, after the clot material toughening, gradually disappear; To swell after shrinking significantly inferior vena cava; Mesenteric vein aggravating gradually; Then see:endothelial cell damage, instead of disappearing and form; Inflammatory cells infiltrating blood vessel walls and thrombus seriously; SD rats in2h,4h,8h,12h,24h,3d,7d and14d is visible thrombosis; Japanese big ear rabbit in4h,8h,12h,24h,3d,7d and14d with thrombosis; The whole pathological evolution conform to the pathological progress of thrombus from acute to chronic phase process;2, SD rats, Japanese big ear rabbit inferior vena cava ligation group and the incidence of DVT of the inferior vena cava stenosis group were significantly higher than control group and normal control group, the building before and after the increase of vascular diameter, length, the thrombus wet weight of the vessel wall, and the corresponding ligation group and narrow group and normal control group with significant differences in the control group (P<0.05), control group no significant difference between the normal control group (P>0.05).3, SD rats:inferior vena cava ligation group2h can be measured the length of thrombosis and thrombosis and corresponding vein wall wet weight,7d peak,14d start value decreases, and21d not measured the corresponding numerical values. Inferior vena cava stenosis group2h can be measured the length of thrombosis and thrombosis and corresponding vein wall wet weight,3d peak,7d start value decreases, and further reduce14d,21d not measured corresponding numerical; Japanese big ear rabbit:inferior vena cava ligation group4h can be measured the length of thrombosis and thrombosis and corresponding vein wall wet weight,7d peak,14d start value decreases, and21d not measured the corresponding numerical values. Inferior vena cava stenosis group4h can be measured the length of thrombosis and thrombosis and corresponding vein wall wet weight,3d peak,7d start value decreases, and further reduce14d,21d not measured value accordingly.3, GP1b alpha protein inferior vena cava stenosis DVT in SD rats model of plasma expression, compared with normal control group and the control group had statistically significant difference (P<0.05); Express the amount in the inferior vena cava stenosis group after8h,24h (P=0.000), the height of the postoperative7d is lower, but still higher than normal control group and control group.[conclusion]1, Successfully established SD rats, Japanese big ear rabbits inferior vena cava ligation, narrow model of DVT. Inferior vena cava stenosis DVT animal models is relatively inferior vena cava ligation method DVT animal model is more suitable for dynamic study of the formation of DVT, peak, fade, In DVT build mould SD rats than Japanese big ear rabbit faster, more economic, easy management, anesthesia for complete thrombosis time is shorter, shorter operation time; But the latter is a smaller field, relatively high fine degree of operation requirements.2, IVC Stenosis model of DVT in rat:2h for incomplete thrombosis,8h can form complete thrombosis, thrombosis peak period of24h～3d,7d～21d for thrombus fade; Japanese big ear rabbit:4h can form incomplete thrombosis,12h can form complete thrombosis,24h～3d for the peak period of thrombus,7d～21d for thrombus subsided.3, GP1b-α is closely related to DVT formation... |
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