Mechanisms Of GLUT1in The Change Of Blood-Brain Barrier Permeability By Methamphetamine And HIV-Tat Protein

Objective:To observe the changes of the blood-brain barrier after administered MA and HIV-Tat, to investigate the mechanisms of GLUT1in the synergies of MA and HIV-Tat on blood-brain barrier. Methods:Male SD rats were ip given MA10mg·kg-1twice daily followed tail intravenous injection given Tat protein0.4μg·kg-1once daily for7days. After the last injection,randomly choose5rats tail intravenous injection given EB for brain tissue EB detective and5rats’brain tissue for protein detective (including2rats for electron microscopy observation) and5rats for immunohistochemical. Results:AS compared with control group, EB of control group were significant lower than test group, suggested blood-brain barrier permeability was increased (P<0.05). MA+Tat group compared with MA and Tat group, level of EB was lower. It implied that MA combine with Tat have synergistic effects on changing permeability of blood-brain barrier. EB in NAC+MA+Tat group is lower than MA+Tat group(P<0.01), suggested NAC can protect BBB from toxicant of MA and Tat. Compared with control group,GLUT1and TJ expression were decreased significantly(P<0.05);MA+Tat group compared with MA and Tat group,changes of GLUT1and TJ have decreased(P<0.01).GLUT1and TJ in NAC+MA+Tat group were higher than MA+Tat group(P<0.01). A series of ultrastructural changes of blood-brain barrier were found under transmission electron-microscopy such as cerebral microvascular endothelial cells became swollen and thinner, the glia and astrocytes also were swollen. Conclusion:MA and Tat protein can change GLUT1expresstion of BBB. And this study show GLUT1participated in the process of MA and HIV-Tat protein synergy change blood-brain barrier function.