Long Chain Non-coding RNA MEG3,ST8SIA3and TUG1Expression In Lung Cancer Research

BackgroundOne of the main lung cancer is a serious hazard to human health, but also the world’s current highest mortality of cancer. Since there is no effective means of early diagnosis of lung cancer, the vast majority of lung cancer in advanced stage when diagnosed clinically, thus resulting in high mortality of lung cancer, so early diagnosis of lung cancer is a key factor in determining the prognosis of patients, and the results of molecular biology research will contribute to the early diagnosis of lung cancer. Like with other human malignancies, lung cancer is the development of oncogene activation results from the interaction of multiple genes tumor suppressor gene inactivation. However, lung cancer, and other factors and the development of the transfer mechanism is very complex, the existing evidence is not sufficient to fully explain the etiology of lung cancer. As the molecular biology and genetic diagnosis technology advances, more and more evidence that there is a close relationship between long-chain non-coding RNA (lncRNA) and the occurrence of cancer, and, as a tumor-specific lncRNA predictors.long chain non-coding RNA (lncRNA) is a transcription of the length of more than200nt of functional RNA molecules, lncRNA many species in the nucleus or cytoplasm to form on a variety of levels of RNA expression levels of genes that regulate epigenetic level, the level of transcription and transcription after the level of regulation of gene expression, the broad participation of almost all the body’s physiological and pathological processes. More and more evidence suggests that abnormalities lncRNA may play an important role in tumorigenesis. lncRNA differentially expressed in normal tissue and tumor tissue and its corresponding atypical hyperplasia, the specificity lncRNA can be used as predictors of tumor, disorders lncRNA DNA methylation can be adjusted in various ways, histone modification, chromatin remodeling and as a precursor miRNA play an important role in tumorigenesis and development. Research on lncRNA will help to understand life on a multi-level expression regulation networks, and is expected to provide a new molecular basis for the prediction of complex diseases, diagnosis, and treatment.ObjectiveBy real time fluorescence quantitative detection of lung cancer and normal tissue adjacent to carcinoma of long chain non-coding RNA MEG3, ST8SIA3and TUG1differentially expressed, target genes predicted its targets, to explore its mechanism and its role in lung cancer development.MethodCollect the third hospital affiliated to kunming medical college in2011-2012hospitalized patients with surgical resection of lung cancer tissue and normal tissue adjacent to carcinoma (cancer cancer from normal tissue at the side of more than5cm), get off after10minutes into the test tube containing RNAlater solution in advance, in the first4℃covered eight hours and then into the-80℃refrigerator for medium and long term.In the postoperative pathological records confirmed that histopathologic types, successfully collected14cases of squamous cell carcinomas of33cases of adenocarcinoma.Respectively to extract total RNA, RNA respectively for SYBR Green fluorescence quantitative PCR, beta actin as internal to detect the relative expression in normal tissue and lung cancer organizations,2-ΔΔCT value in the organization relative to express, using t test, Spearman rank correlation analysis of relative expression of RNA expression and TNM staging and the correlation between immunohistochemistry.Result1) a total of47cases of sample collection, its basic situation:people are the han nationality;Organization type33cases of adenocarcinoma, squamous carcinoma (14;Minimum age for maximum71,32,average54.91+/-9.62；Gender is male32cases of f-emale15cases；Smoking in29cases (62%)；Drinking22cases (47%)；Differentiation is:low differentiation18cases,28cases of differentiation,high differentiation in1case；GST PI:5cases of(-),15cases(+),9cases(++),3cases(+++)；PgP:7cases of(-),14cases of(+),9cases(++),2cases(+++),TOPO II:6cases(一),20cases of(+),6cases(++)；LRP:2cases of(-),14cases of(+),13cases (++),3cases(+++),MRP:4cases of(一),13cases of(+),15cases(++)；P53:9cases of(-),15cases(+),7cases(++),1case(+++)；The average Ki67:5%,5%and90%.2)MEG3relative expression in the cancer tissue volume significantly lower than the normal tissue adjacent to carcinoma(P<0.05)；3)TUG1relative expression in the cancer tissue volume significantly lower than the normal tissue adjacent to carcinoma(P<0.05)；3)ST8SIA3relative expression in the cancer tissue and normal tissue adjacent to carcinoma in statistical difference(P>0.05)；4)ST8SIA3and TOPO Ⅱ negative correlation(r=0.47,P=0.47).5)TUGl and TOPO Ⅱ negative correlation(r=0.42,P=0.42).6)ST8SIA3and lymphatic metastasis(N)into positive correlation(r=0.29,P=0.29).Conclusion1)MEG3,TUG1in lung cancer tissues compared to adjacent normal tissues was significantly lower, the difference was statistically significant, suggesting the development process from the role of tumor suppressor genes MEG3,TUG1may occur in the lung;2) ST8SIA3expressed in lung cancer and normal tissue adjacent to carcinoma,there were no significant differences with TNM staging of lymph node metastases is closely relative to (N),and clinical indicators TOPO Ⅱ negative correlation,and provide a clue for future ST8SIA3research in lung cancer.