Pathogenesis Research Study In Melasma

Objective:To research the function of melanogenesis, skin barrier, inflamation factors and vascular factors in melasma.Methods:1.Skin lesions of melasma patients from the clinic were taken as study group. Normal skin surrounding the lesions in patients with Nevi was taken as control group. The expression and distribution of TYR, TRP-1, TRP-2,K6, K10, K14, Filaffrin, Involucrin, Loricrin, ASAH1, PGE2,SCF, ET-1, TLR2, TLR4, CD34, VEGF in the lesions were detected by immunohistochemical study, and no significance difference in HMB45.S100. RT-PCR was performed to measure the expression of TLR2and TLR4mRNA.2. The blood sample of clinic melasma patients was taken as study group. Blood of normal people from the medical examination center was taken as controlled group. RT-PCR was performed to measure the expression of TLR2and TLR4mRNA in the blood sample of each group. The whole process should be ruled out diseases of other system.T-test was used for statistics and P<0.05was defined as significant.Results:Ten lesions of melasma patients and ten lesions of normal controlled skin sample were included from the clinic. Immunohistochemistry showed significant difference of TYR, TRP-1, TRP-2, HMB45, S100, K6, K10, K14, Filaffrin, Involucrin, Loricrin, ASAH1, PGE2, SCF, TLR2, TLR4, CD34, VEGF expression in each group (P<0.05). HMB45and S100only expressed in epidermis in both group. RT-PCR study showed significant difference of the TLR2and TLR4expression in each group (P<0.05), which was significantly higher in the melasma group.40melasma blood samples were collected in the clinic and40normal control blood samples collected in the medical examination center. No significant difference was found in the expression of TLR-2and TLR-4of both groups (P>0.05). Conclusion:1.The melanogenesis, skin barrier, inflamation factors and vascular factors played a significant influence on melasma. Melanocyte only located in epidermis. Local skin Toll receptor-mediated immune responses might be related to the initiation of melasma.