The Effection Of Nicotinamide Mononucleotide Adenylyl Transferase1to Catalase And Tyrosine Hydroxylase In Cell Models Of Parkinson’s Disease (PD) MES23.5Cells

Aim To invest the relativation between the expression of catalase (CAT) and Tyrosine hydroxylase (TH) in cell Models of Parkinson’s disease (PD) MES23.5cells with nicotinamide mononucleotide adenylyl transferase1(NMNAT1).Methods Before Parkinsonian cell model establishment, synthetic NMNAT1overexpression(NMNAT1-OE) vehicle plasmids, NMNAT1overexpression plasmids, NMNAT1siRNA vehicle plasmids and NMNAT1siRNA (NMNAT1RNAi) plasmids were transfected into MES23.5cells with cell transfection techniques. The cell Models were divided into six groups:pEGFP group, pEGFP+MPP+group, NMNAT1-OE+MPP+group, pMagic4.1, pMagic4.1+MPP+, NMNAT1-RNAi+MPP+,The protein level of Caspase8and TH in the six groups were measured with Western blots.Results Compared with pEGFP group, marked decreased in the protein level of CAT was observed in pEGFP+MPP+group (t=9.01, P＜0.001),and TH expression decreased (t=0.32,p=0.012). Compared with pEGFP+MPP+group, CAT expression increased (t=2.51, p=0.033) and TH expression increased (t=0.45,p=0.002) in NMNAT1-OE+MPP+group. Compared with pMagic4.1control group, marked decrease in the protein level of CAT (t=2.47, p=0.036) and decrease in the protein level of TH (t=0.49, p=0.001) was observed in pMagic4.1+MPP+group, Compared with pMagic4.1+MPP+group, CAT expression decrease(t=3.53, p=0.006) and TH expression decreased (t=0.39,p=0.004) in NMNAT1-RNAi+MPP+group.Conclusion Our result suggests that NMNAT1is effective to up-regulate the expression of CAT which is one of antioxidant enzyme against oxidative injury and TH. Nmnatl may play an important role in protecting DA neurons through CAT.