Chromosomal Karyotype Analysis And Clinical Prognosis Study Of Myelodysplastic Syndromes

Objective:To investigate the characterizations of chromosomal karyotype in patients with myelodysplastic syndromes (MDS), explore the relationship between different subtypes with abnormal chromosome karyotypes and clinical prognosis.Methods:142patients diagnosed with MDS were consecutively collected and classified according to the WHO classification standards, while the chromosome was prepared with brief culture of bone marrow, and the karyotype was analysed by R banding. Clinical information of patients were grouped by International Prognosis Scoring System(IPSS) and each two groups were compared survival rate.Results:Among142MDS patients,76cases were found with chromosomal abnormal karyotype, the abnormal detection rate was53.5%. Each subtype of abnormal karyotype detection rates showed no statistical significant difference (P>0.05).+8(12.0%)、20q-(5.6%) were common abnormal karyotype, only one case of5q-.; dup(1q)、i(17q)、i(8q、i(lq)、der(17;18)、dic(12;20) were rare abnormal karyotype, and balanced translocations included t(3;21)/t(10;11)、t(1;3)、t(6;9)、t(8;22)/t(11;12)、t(5;17)、 t(3;3)、der(12)t(1;12). der(11)t(1;11)、der(12)t(12;14). The rate of complex abnormalities in RAEB-2was higher than other subtypes and had statistical difference with RCMD (χ2=5.253, P=0.022).The patients with abnormal chromosome12had13cases (9.2%) and were mainly distributed in RAEB.The proportion of each other two subtype in IPSS classification had statistical difference(P<0.001) excluded the proportion between RA and RARS. There was significant difference in survival rate between RAEB-2with RARS(P=0.01)、RCMD(P=0.009)and RAEB-1(P=0.034).Conclusion:Karyotype analysis in combination with morphological examination has important value for diagnosis and evaluating prognosis of MDS. The abnormal chromosome12may be a concomitant distortion after MDS common distortion in the process of disease progression.