Effects Of Adrenergic Receptor-β2on The Liver And Skeletal Muscle Of Rat With Hemorrhagic Shock In Expressing PGC-1

Objective:The purpose of this study is to investigate the adrenergic receptor-β2regulation ofhemorrhagic shock in rats liver and skeletal muscle PGC-1expression and prognosisMethod:102male rats built the hemorrhagic shock-recovery model (calculated bloodlettingnumber according to the weight of22.5ml/kg, finishing in10minutes and maintaining30minutes). They were divided into three groups randomly---respectively contrastgroup(sham), shock group(SR), interention group(PR). Contrast group hadn’t anyoperations; shock group built hemorrhagic shock model and30minutes later began toresuscitate; interention group immediately gave ICI118.551by vein after buildinghemorrhagic shock model and then30minutes later resuscitated. Observe and recordhemodynamic changes of rats from all groups: mean arterial pressure(MAP) and heartrate(HR). Record the survival time and calculate the72-hour survival rate of rats. Usingquantitative PCR and Western blotting method to detect the2hours,4hours,8hoursand24hours the liver and skeletal muscle PGC-1mRNA and protein expressionchanges.Result:1.Survival rate:The survival rate of rats of three groups is respectively100percent,50percent and80percent. The average survival time is respectively72.00±0.00h,48.00±8.25h,62.40±3.40h. There were significant differences between the shock andintervention groups (p<0.05).2.Hemodynamic: Shock rats MAP changes in the intervention group at each time pointwas no significant difference (p<0.05). Shock rats HR at all time points changes in the intervention group was not statistically significant (p<0.05).3.Expression change of hepatic and skeletal muscle tissue PGC-1mRNA: Theexpression of PGC-1mRNA of interention group is less than that of shock groupsignificantly at2hours point. The difference of two groups has statisticalsignificance(p<0.05). That of shock group and interention group has no diffenrence atother time points(p>0.05).4.Expression change of the PGC-1protein of hepatic and skeletal muscle tissue: Theexpression of PGC-1of interention group is less than that of shock group significantlyat2hours point. The difference of two groups has statistical significance(p<0.05). Thatof shock group and interention group has no diffenrence at other time points(p>0.05).Conclusion:1.Blocking adrenergic receptor-β2can improve the72-hour survival rate of rats withhemorrhagic shock.2.Blocking adrenergic receptor-β2receptors may be shortly downregulate the expressionof PGC-1gene and protein of hepatic tissue and skeletal muscle tissue of rats withhemorrhagic shock.3.Blocking adrenergic receptor-β2may downregulate the expression of PGC-1toreduce the energy consumption of hemorrhagic shock in rats, and thus improvesurvival.