Effect Of Baicalin On Endoplasmic Reticulum Stress Tunicamycin-induced Injury In Cultured Neonatal Rat Cardiomyocytes

Chinese herbal medicine has a history of several thousand years, is China’s importantnatural products, the existing species has amounted to12807. In recent years, as chemistry,biology, pharmacology and pharmaceutical analysis to study the progress of modernscience, such as research and development of multidisciplinary, multiple technology oftraditional Chinese medicine (TCM) has been a long-term development. Especially a largeamount of Chinese herbal medicine composition separation, purification, drug effect anddrug metabolism study progress, and a variety of traditional Chinese medicine monomerand complex components are widely used in the treatment of clinical disease, plays a veryimportant pharmacological effects. Preparation and research and development oftraditional Chinese medicine monomer, therefore, become the developmental trend ofmodern Chinese medicine, how to select from a large number of Chinese native medicineingredient has good pharmacological effects of monomer composition, is the importantdirection of modern drug discovery. And has been confirmed with traditional Chinese medicine monomer supply and reduce ischemic injury, improve heart antiapoptotic effectEndoplasmic reticulum (ER) is an important organelles eukaryotic cells, whichconsists of a closed membrane system and cavity form reticular structure of communicatewith each other, participated in the nascent peptide chain folding, protein synthesis andtransport, and other functions. The study found that glucose energy shortage, viralinfections and other stimulus will affect the steady state of endoplasmic reticulum,violation of protein processing and a calcium loss. Clothes toxin, the accumulation ofcholesterol by endoplasmic reticulum stress balance of endoplasmic reticulum destructionapoptosis. In ischemia-reperfusion injury, atherosclerosis, hypertension and cardiovasculardiseases such as myocardial infarction and heart failure were the participation of ER stress.Endoplasmic reticulum stress activated, a molecular chaperone GRP78highly expression,the expression of CHOP and phosphorylation JNK cause cell apoptosis. Baicalin (BC) is akind of flavonoids, as a kind of commonly used Chinese herbal monomer compounds. BCwith antioxidant, anti apoptotic cells, anti-inflammatory and other protection. BC caninhibit the occurrence of ERS work? Has not been reported. To this end, we assume thatthe endoplasmic reticulum stress in the process of the myocardial cell apoptosis mediatedby baicalin may also play an important role. Based on the study of this study was to earlyto application of high flux membrane chromatography to observe the effect of traditionalChinese medicine monomer baicalin, and on the basis of the study the garment toxininduced by endoplasmic reticulum stress the role of myocardial cell injury and possiblemechanism.Aims:1.Observe whether the baicalin can Combine cell membrane recepto by cellmembrane chromatography2.Discuss the the effect of myocardial cell damage in the Baicalin-inducedendoplasmic reticulum stress.Methods:1.The cell culture and grouping of the experiments: the primary cultured neonatalrat cardiomyocytes were separated into four groups: the culeture solution group (ctrl), the Tm treatment group (Tm), and the Tm and baicalin treatment group (Tm)，the baicalintreatment group (BC)2.The biological affinity chromatography: observed whether baicalin can effectsbiological membrane on cell membrane chromatography3.The cell molecular level: The myocardial cell survival rate determined by MTT，and LDH test cell damage, TUNEL apoptosis detection, Western blot detection CHOPprotein levelsResult:1.The use of biological affinity chromatography, membrane chromatography in themobile phase for water flow velocity under the condition of0.2mL/min, DAD detected bytraditional Chinese medicine monomer baicalin can apply to cell membrane receptor.2.Tm can damage the myocardial cell in time and the concentration dependence ofthe myocardial cell survival time point significant difference (P <0.05); Tm significantlyincreased the endoplasmic reticulum stress CHOP expression and apoptosis TUNEL level.3.Compared with Tm group,50mu M/mL of BC can significantly inhibit the Tm ofmyocardial cell injury, increase myocardial cell survival rate (P <0.05); At the same time,the BC can significantly inhibit the Tm cause myocardial cell endoplasmic reticulumstress damage, make the CHOP expression and apoptosis of TUNEL level significantlylowered (P <0.05).Conclusion:1.The traditional Chinese medicine monomer baicalin can be applied to cells, and cellmembrane receptor.2.Garment toxin can induce myocardial cell endoplasmic reticulum stress damage,leading to cell apoptosis; Treatment of baicalin, can reduce the clothes geneticallymediated myocardial cell damage, to protect the cells.